Investigation of the effects of some pesticides on carbonic anhydrase isoenzymes

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-08-08 DOI:10.1002/jmr.3048

Aybike Baltacı, Kubra Cıkrıkcı, Nahit Gençer

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Abstract

The aim of this study was to investigate the inhibitory effects of some pesticides known to have harmful effects on human health on carbonic anhydrase isoenzymes. Therefore, carbonic anhydrase isoenzymes (hCA I and II) were purified from human erythrocytes. The isoenzymes were purified from human erythrocytes by using an affinity column that has the chemical structure of Sepharose-4B-4-(6-amino-hexyloxy)-benzenesulfonamide. The purity of the isoenzymes was checked by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE). It was determined that the pesticides used in this study inhibit hCA I and hCA II isoenzymes at different levels in vitro. It was determined that the strongest inhibitor for the hCA I enzyme was Carbofuran (IC₅₀:6.52 μM; K_i: 3.58 μM) and the weakest one was 1-Naphtol (IC₅₀:16.55 μM; K_i: 14.4 μM) among these pesticides. It was also found that the strongest inhibitor for the hCA II enzyme was coumatetralil (IC₅₀:5.06 μM; K_i: 1.62 μM) and the weakest one was Dimethachlor (IC₅₀ 14.6 μM; K_i: 8.44 μM).

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几种农药对碳酸酐酶同工酶影响的研究

本研究的目的是探讨一些已知对人体健康有害的农药对碳酸酐酶同工酶的抑制作用。因此，从人红细胞中纯化了碳酸酐酶同工酶(hCA I和II)。用具有Sepharose-4B-4-(6-氨基-己基氧基)-苯磺酰胺化学结构的亲和柱从人红细胞中纯化了同工酶。用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDSPAGE)检测同工酶的纯度。结果表明，本研究使用的农药在体外对hCA I和hCA II同工酶均有不同程度的抑制作用。结果表明，对hCA I酶抑制作用最强的是Carbofuran (IC50:6.52 μM;Ki: 3.58 μM)，最弱的是1-萘酚(IC50:16.55 μM;Ki: 14.4 μM)。同时发现，对hCA II酶的抑制作用最强的是香豆素(IC50:5.06 μM;Ki: 1.62 μM)，最弱的是二甲草胺(IC50: 14.6 μM;Ki: 8.44 μM)。

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