Ginsenoside Rc attenuates myocardial ischaemic injury through antioxidative and anti-inflammatory effects.

IF 3.9 3区医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pharmaceutical Biology Pub Date : 2022-12-01 DOI:10.1080/13880209.2022.2072518

Lei Shi, Wenwen Fu, Huali Xu, Shihui Li, Xinyu Yang, Wei Yang, Dayun Sui, Quanwei Wang

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引用次数: 7

Abstract

Context: Panax ginseng C. A. Meyer (Araliaceae) is a famous Asian medicine. Ginsenoside Rc is a component isolated from Panax ginseng.

Objective: This study evaluates the effect of ginsenoside Rc on myocardial ischaemic injury.

Materials and methods: Male Swiss mice were subcutaneously injected with 50 mg/kg isoproterenol once a day for three days. Ginsenoside Rc (10, 20, or 40 mg/kg) was intragastrically administered 1 h after isoproterenol injection. The mice in the control group were subcutaneously injected with normal saline and intragastrically given 0.5% CMC-Na. CK-MB and troponin T were assayed. Histopathological examination of myocardium was conducted. The expression of Nrf2, GCLC, GCLM and HO-1 in heart tissues was evaluated by Western blot.

Results: In myocardial ischaemic mice, ginsenoside Rc reduced the levels of CK-MB (197.1 ± 15.7, 189.9 ± 19.0, 184.0 ± 14.4 vs. 221.6 ± 27.9) and troponin T (10.3 ± 1.7, 9.5 ± 1.3, 8.7 ± 1.7 vs. 13.4 ± 2.4). Ginsenoside Rc attenuated the necrosis and inflammatory cells infiltration in myocardium. Furthermore, ginsenoside Rc not only decreased the contents of MDA, TNF-α but also increased GSH level in the heart tissues. The expression of Nrf2, GCLC, GCLM and HO-1 was significantly increased in the animals treated with ginsenoside Rc. ML385, an Nrf2 inhibitor, blocked partially the ginsenoside Rc-mediated cardioprotective effect. Ginsenoside Rc attenuated myocardial ischaemic injury in mice, which may be, in part, through its antioxidative and anti-inflammatory effects.

Conclusions: This study indicated that ginsenoside Rc might be a novel candidate for treatment of myocardial ischaemia.

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人参皂苷Rc通过抗氧化和抗炎作用减轻心肌缺血损伤。

背景:人参是一种著名的亚洲药材。人参皂苷Rc是从人参中分离得到的一种化学成分。目的:探讨人参皂苷Rc对心肌缺血损伤的影响。材料与方法:雄性瑞士小鼠皮下注射异丙肾上腺素50 mg/kg，每天1次，连续3天。异丙肾上腺素注射后1 h ig人参皂苷Rc(10、20或40 mg/kg)。对照组小鼠皮下注射生理盐水，灌胃0.5% CMC-Na。检测CK-MB和肌钙蛋白T。行心肌组织病理学检查。Western blot检测心肌组织中Nrf2、GCLC、GCLM和HO-1的表达。结果:人参皂苷Rc降低心肌缺血小鼠CK-MB(197.1±15.7,189.9±19.0,184.0±14.4比221.6±27.9)和肌钙蛋白T(10.3±1.7,9.5±1.3,8.7±1.7比13.4±2.4)水平。人参皂苷Rc可减轻心肌坏死和炎症细胞浸润。人参皂苷Rc不仅能降低大鼠心脏组织中MDA、TNF-α含量，还能提高GSH水平。人参皂苷Rc处理后，Nrf2、GCLC、GCLM和HO-1的表达均显著升高。Nrf2抑制剂ML385部分阻断了人参皂苷rc介导的心脏保护作用。人参皂苷Rc减轻小鼠心肌缺血损伤，这可能部分是通过其抗氧化和抗炎作用。结论:人参皂苷Rc可能是治疗心肌缺血的新候选药物。

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来源期刊

Pharmaceutical Biology 医学-药学

CiteScore

6.70

自引率

2.60%

发文量

191

审稿时长

1 months

期刊介绍： Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine. Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.