Effect of polyols on membrane structures of liposomes: A study using small-angle X-ray scattering data and generalized indirect Fourier transformation

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Chemistry and Physics of Lipids Pub Date : 2022-11-01 DOI:10.1016/j.chemphyslip.2022.105253

Aika Sekine , Taku Ogura , Koji Tsuchiya , Kaoru Ohishi , Yuji Masubuchi , Masaaki Akamatsu , Kenichi Sakai , Masahiko Abe , Hideki Sakai

{"title":"Effect of polyols on membrane structures of liposomes: A study using small-angle X-ray scattering data and generalized indirect Fourier transformation","authors":"Aika Sekine , Taku Ogura , Koji Tsuchiya , Kaoru Ohishi , Yuji Masubuchi , Masaaki Akamatsu , Kenichi Sakai , Masahiko Abe , Hideki Sakai","doi":"10.1016/j.chemphyslip.2022.105253","DOIUrl":null,"url":null,"abstract":"<div>This study aimed to evaluate the membrane structure of distearoylphosphatidylcholine (DSPC) liposomes dispersed in water containing various types of polyols with low molecular weight such as glycerin (Gly), 1,3-butandiol (BG), and propylene glycol (PG). To clarify the detailed membrane structure, generalized indirect Fourier transformation (GIFT) analysis, which provides information about the bilayer spacing, bilayer thickness, number of lamellar layers, and membrane flexibility, was applied to small-angle X-ray scattering (SAXS) data of the present system. The GIFT results showed that the bilayer thickness of the DSPC liposomes followed the order Gly>>BG>PG. In addition, the membrane flexibility estimated by the Caille parameter was in the order Gly>>BG>PG; this result was supported by the gel-liquid crystal phase transition temperature (Tc) obtained by differential scanning calorimetry (DSC). These results, together with the Raman spectra, suggest that BG and PG incorporated into the bilayers of DSPC liposomes result in the formation of an interdigitated lamellar structure.</div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry and Physics of Lipids","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0009308422000810","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 1

Abstract

This study aimed to evaluate the membrane structure of distearoylphosphatidylcholine (DSPC) liposomes dispersed in water containing various types of polyols with low molecular weight such as glycerin (Gly), 1,3-butandiol (BG), and propylene glycol (PG). To clarify the detailed membrane structure, generalized indirect Fourier transformation (GIFT) analysis, which provides information about the bilayer spacing, bilayer thickness, number of lamellar layers, and membrane flexibility, was applied to small-angle X-ray scattering (SAXS) data of the present system. The GIFT results showed that the bilayer thickness of the DSPC liposomes followed the order Gly>>BG>PG. In addition, the membrane flexibility estimated by the Caille parameter was in the order Gly>>BG>PG; this result was supported by the gel-liquid crystal phase transition temperature (T_c) obtained by differential scanning calorimetry (DSC). These results, together with the Raman spectra, suggest that BG and PG incorporated into the bilayers of DSPC liposomes result in the formation of an interdigitated lamellar structure.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

多元醇对脂质体膜结构的影响:基于小角x射线散射数据和广义间接傅里叶变换的研究

本研究旨在评价二硬脂酰磷脂酰胆碱(dsc)脂质体在含有甘油(Gly)、1,3-丁二醇(BG)和丙二醇(PG)等各种低分子量多元醇的水中分散的膜结构。为了明确膜的详细结构，将广义间接傅立叶变换(GIFT)分析应用于该体系的小角度x射线散射(SAXS)数据，该分析提供了有关双层间距、双层厚度、层数和膜柔韧性的信息。GIFT结果显示，DSPC脂质体的双层厚度遵循Gly>>BG>PG的顺序。此外，Caille参数估计的膜柔韧性在Gly>>BG>PG;差示扫描量热法(DSC)测得的凝胶-液晶相变温度(Tc)支持了这一结果。这些结果与拉曼光谱一起表明，BG和PG结合到dsc脂质体的双层中导致形成交错的片层结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.