Wai Yin Yau, Dr. Samuel Kin-Man Lai, Dr. Pilar Blasco, Prof. Xuechen Li, Prof. Kwan Ming Ng, Dr. Chun Nam Lok, Dr. Ho Yu Au-Yeung
{"title":"Activity-based Fluorescent Imaging of Alcohol Dehydrogenase Activity in Living Cells","authors":"Wai Yin Yau, Dr. Samuel Kin-Man Lai, Dr. Pilar Blasco, Prof. Xuechen Li, Prof. Kwan Ming Ng, Dr. Chun Nam Lok, Dr. Ho Yu Au-Yeung","doi":"10.1002/anse.202300012","DOIUrl":null,"url":null,"abstract":"<p>Development of a fluorescent probe for activity-based sensing of activity of alcohol dehydrogenase, a key enzyme in ethanol biooxidation, is reported. A caged coumarin reporter is released upon the selective oxidation by the enzyme with a strong, >60-fold emission enhancement. The probe has a low cytotoxicity and has been applied in visualising alcohol dehydrogenase activity in HepG2, A549 and HEK293T cells, demonstrating its potential as a convenient, easy-to-use bioanalytical tools in unveiling the roles of the enzyme in alcohol metabolism.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analysis & sensing","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/anse.202300012","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
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Abstract
Development of a fluorescent probe for activity-based sensing of activity of alcohol dehydrogenase, a key enzyme in ethanol biooxidation, is reported. A caged coumarin reporter is released upon the selective oxidation by the enzyme with a strong, >60-fold emission enhancement. The probe has a low cytotoxicity and has been applied in visualising alcohol dehydrogenase activity in HepG2, A549 and HEK293T cells, demonstrating its potential as a convenient, easy-to-use bioanalytical tools in unveiling the roles of the enzyme in alcohol metabolism.
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