Molecular Identification of Phosphate-Solubilizing Yeast Isolate KR.1BP.4 From Citatah Karst Area

Abstract

Citatah Karst Area has diverse microorganisms, including phosphatesolubilizing yeasts. Phosphate-solubilizing yeasts in the soil play an important role in solubilizing organic phosphates into inorganic ones absorbable by plants for photosynthetic process and root development. Phenotypical and biochemical identification of phosphate-solubilizing yeast isolate KR.1BP.4 has been reported, but molecular identification should be performed to ensure accurate results. The aim of this research was to identify the species of phosphate-solubilizing yeast isolate KR.1BP.4. This research was carried out by a descriptive method using the following ITS primers: forward primer ITS1(5’-TCC GTA GGTGAACCTGCG G-3’) and reverse primerITS4 (5’-TCCTCCGCT TAT TGA TAT GC-3’). The method consisted of three stages, namely extraction with Genomic DNA Mini Kit (Blood and Cultured cell), PCR with KAPA2G Robusta PCR MasterMix, and electrophoresis and sequencing. Data analysis was performed using BLAST, and alignment was performed using MEGA 6.0. The phosphate-solubilizing yeast isolate KR.1BP.4 was identified as Trichosporonasahiiwith an ITS fragment length of 774 bp. Based on the phylogenetic tree and genetic distance, the T. asahiiisolate KR.1BP.4 had the closest relatedness with T. asahiistrain V9, T. asahii strain V3, T. asahiiYCH116, T. asahiiKDLYL 4-1, T. asahiiV1, T. asahiiAP.MSU6, and T. asahii PMM08-1100L at a distance value of 0%. From this research, it can be concluded that the isolate KR.1BP.4 belongs to the species Trichosporonasahii.