Evaluation of Different Fungicides against Colletotrichum graminicola, the Cause of Red Leaf Spot of Sorghum

Misbah Ali, Safdar Ali, M. Zeshan, R. Binyamin, N. Ahmed, Muhammad Usman Ghani, Awais Khan
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Abstract

| Sorghum (Sorghum bicolor L. Moench) is a very important cereal crop for human beings and livestock. It is subjected to many diseases in Pakistan like fungal, bacterial, and viral among which the fungal diseases are the most destructive. Colletotrichum graminicola is the causal organism of red leaf spot of sorghum which causes heavy losses to the sorghum crop cultivated in different areas of Pakistan. It produces black anthracnose spots on all the above ground parts especially leaves. In the present study, four fungicides (Aliette, Cabrio Top, Nativo, and Melody Duo) were evaluated against C. graminicola under in-vitro and in-vivo conditions. The poisoned food technique was used for in vitro trials with three concentrations (150 ppm, 200 ppm, and 250 ppm). The most effective concentration of fungicides from laboratory experiment was used in field conditions against red leaf spot of sorghum. Results showed that Cabrio Top was the most effective fungicide by showing 88% fungal inhibition while Native also exhibited good results by showing 78.1% fungal inhibition. The remaining 2 fungicides Melody Duo and Aliette were least effective by showing 48.2% and 37.5% fungal inhibition, respectively. In vivo experiment from all four fungicides, Cabrio Top and Nativo showed best results by controlling 74.57% and 71.52%, respectively disease over control while Melody Duo and Aliette were least effective by controlling 65.31% and 60.08% disease over control. It may be concluded that the Cabrio Top at 250 ppm concentration is the best to manage red leaf spot disease severity under field conditions.
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不同杀菌剂对高粱红叶病病原菌谷草炭疽病的防治效果评价
高粱(Sorghum bicolor L. Moench)是人类和牲畜非常重要的谷类作物。它在巴基斯坦遭受许多疾病,如真菌、细菌和病毒,其中真菌疾病是最具破坏性的。炭疽杆菌是引起高粱红叶病的病原菌,对巴基斯坦不同地区的高粱作物造成重大损失。它在所有地上部分尤其是叶子上产生黑色炭疽病斑。在体外和体内条件下,研究了4种杀菌剂(Aliette、Cabrio Top、Nativo和Melody Duo)对禾草弧菌(C. graminicola)的抑制作用。中毒食品技术在体外试验中使用了三种浓度(150 ppm、200 ppm和250 ppm)。在田间条件下,采用室内试验得到的最有效杀菌剂浓度防治高粱红叶病。结果表明,Cabrio Top的抑菌效果最好,抑菌率为88%;Native的抑菌率为78.1%。其余2种杀菌剂的抑菌效果最差,分别为48.2%和37.5%。4种杀菌剂体内试验中,Cabrio Top和Nativo的防病效果最好,分别为74.57%和71.52%,而Melody Duo和Aliette的防病效果最差,分别为65.31%和60.08%。结果表明,在田间条件下,施用250 ppm浓度的卡布里奥玉米对红叶病的防治效果最好。
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