Grace E. Houston, Allison K. Blomme, O. Harrison, J. Bai, J. Woodworth, Cassandra K. Jones, Elizabeth G. Poulsen-Porter, C. Paulk, J. Gebhardt
{"title":"Evaluating the impact of organic matter and sample processing technique on RNA detection using environmental samples","authors":"Grace E. Houston, Allison K. Blomme, O. Harrison, J. Bai, J. Woodworth, Cassandra K. Jones, Elizabeth G. Poulsen-Porter, C. Paulk, J. Gebhardt","doi":"10.54846/am2022/106","DOIUrl":null,"url":null,"abstract":"This study evaluated sample processing methods and the presence of organic matter on detection of porcine epidemic diarrhea virus (PEDV) from environmental samples using real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Steel coupons were inoculated with PEDV and different types of organic material contamination. Surface samples were collected and processed in one of four ways: none, centrifugation, syringe filtration, or combination of centrifugation and syringe filtration, then submitted for PEDV qRT-PCR. There was a surface inoculation type by processing method interaction (P < .001) that impacted the sample cycle threshold value. Centrifugation resulted in the most consistent detection of PEDV RNA.","PeriodicalId":120947,"journal":{"name":"AASV Annual Meeting","volume":"10 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"AASV Annual Meeting","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54846/am2022/106","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
This study evaluated sample processing methods and the presence of organic matter on detection of porcine epidemic diarrhea virus (PEDV) from environmental samples using real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Steel coupons were inoculated with PEDV and different types of organic material contamination. Surface samples were collected and processed in one of four ways: none, centrifugation, syringe filtration, or combination of centrifugation and syringe filtration, then submitted for PEDV qRT-PCR. There was a surface inoculation type by processing method interaction (P < .001) that impacted the sample cycle threshold value. Centrifugation resulted in the most consistent detection of PEDV RNA.
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