The Influence of Panax Notoginseng Saponins and Tripterygium Glycosides on the TNF-1, IL-6, PAI-1 Level in CIA Rat Peripheral Blood and the MCP-1 Expression in Myocardium

Abstract

Objective: To observe the influence of Panax Notoginseng Saponins and tripterygium glycosides on the TNF, IL-6, PAI-1 level in CIA rat peripheral blood and the MCP-1 expression in myocardium. Methods: randomly dividing the successfully copied CIA rats into model, TG(10mg/Kg.d), PNS(200mg/Kg.d)+TG(10mg/Kg.d), MTX(1.6mg/Kg.w) group and Control group was set. Drawing blood after a six-week administration, opening the chest to take out the heart. The liquid phase protein chip technology was used to detect the content of serum TNF, IL-6, PAI-1, and immunohistochemical method to detect the myocardial MCP-1 protein expression. Results: Compared with the control group, the rat serum TNF, IL-6, PAI-1 level in CIA model group is obviously increased, the myocardial MCP-1 protein expression is significantly up-regulated, TG and PNS can obviously reduce the TNF, IL-6, PAI-1 level in serum, improve the pathological state of myocardium, and down-regulate the expression of MCP-1 in myocardium, and its effect is significantly better than that of TG and MTX group. Conclusions: the effective components of Chinese herbal medicine TG combined with PNS has a significant role in the prevention and treatment of cardiovascular injury, its mechanism may be related to reducing serum TNF, IL-6, PAI-1 and MCP-1 expression in myocardium to exert anti-inflammatory effect.