G. Salzman, J. M. Crowell, B. J. Price, K. M. Hansen, M. Ingram, P. Mullaney
{"title":"Scattering techniques to detect and record various human cell types","authors":"G. Salzman, J. M. Crowell, B. J. Price, K. M. Hansen, M. Ingram, P. Mullaney","doi":"10.1364/cleos.1976.wh2","DOIUrl":null,"url":null,"abstract":"Light-scattering techniques represent an appealing approach to the flow-system analysis and sorting of mammalian cells because they do not require fixation and staining, procedures that kill the cells and can introduce artifacts. A flow-system instrument has been developed1,2 at the Los Alamos Scientific Laboratory in which the intensity of light scattered by a cell as it passes through a focused laser beam is measured simultaneously at up to 32 angles between 0° and 21° with respect to the laser beam, and the scatter pattern is transferred to a computer for analysis.","PeriodicalId":301658,"journal":{"name":"Conference on Laser and Electrooptical Systems","volume":"40 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Conference on Laser and Electrooptical Systems","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1364/cleos.1976.wh2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Light-scattering techniques represent an appealing approach to the flow-system analysis and sorting of mammalian cells because they do not require fixation and staining, procedures that kill the cells and can introduce artifacts. A flow-system instrument has been developed1,2 at the Los Alamos Scientific Laboratory in which the intensity of light scattered by a cell as it passes through a focused laser beam is measured simultaneously at up to 32 angles between 0° and 21° with respect to the laser beam, and the scatter pattern is transferred to a computer for analysis.
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