Isolation of Bacteria From Seahorse (Hippocampus barbouri)

Indra Gandhi Ahmad, H. Anshary, S. Sriwulan
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Abstract

Captive breeding of seahorses (Hippocampus barbouri) which is a follow-up of their activities in the exploitation of natural large - scale does not escape from the problems of the disease. Gills and kidneys are the main target of the inspection. Wet mount is one way of checking the disease. This study aims to look at other forms of bacteria isolated from the gills and kidney seahorse (H. barbouri) morphologically. Media used are media Zobell Marine Agar (ZMA) and Thiosulphate Citrate Bile Salts Sucrose Agar (TCBSA) then be isolated bacteria with a pillowcase technique spread to see koloi dominant bacteria. more dominant bacteria grow dimurinikan back with scratch method (streak). The result of five isolates (isolates A, B, C, D and E) were predominantly found growing on media ZMA, two isolates (isolates B and C) not found growing on media TCBSA. Overall bacteria in media and TCBSA ZMA has the form round colonies and smooth-edged. TCBSA media can not be found on the bacterial colonies that fluoresce.
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海马体细菌的分离
海马(Hippocampus barbouri)的圈养繁殖是其自然大规模开发活动的后续,并没有逃避疾病的问题。鳃和肾是检查的主要目标。湿片是检查疾病的一种方法。本研究旨在观察从鳃和肾海马(H. barbouri)形态学上分离的其他形式的细菌。培养基为佐贝尔海洋琼脂(ZMA)和硫代硫酸柠檬酸胆盐蔗糖琼脂(TCBSA),然后用枕套技术分离细菌传播,观察koloi优势菌。更占优势的细菌生长dimurinikan背部与划痕法(条纹)。结果菌株A、B、C、D和E在ZMA培养基上主要生长,菌株B和C在TCBSA培养基上未见生长。培养基和TCBSA ZMA中的细菌菌落总体呈圆形,边缘光滑。在荧光菌落上找不到TCBSA培养基。
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