D. Bout, H. Dupas, M. Capron, A. El Gazawi , Y. Carlier, A. Delacourte , A. Capron
{"title":"Purification, immunochemical and biological characterization of malate dehydrogenase ofSchistosoma mansoni","authors":"D. Bout, H. Dupas, M. Capron, A. El Gazawi , Y. Carlier, A. Delacourte , A. Capron","doi":"10.1016/0161-5890(78)90036-6","DOIUrl":null,"url":null,"abstract":"<div><p>Affinity chromatography with AMP-Sepharose allows us to purify malate dehydrogenase of<em>Schistosoma mansoni</em> and to perform the studies about the immunochemical and biological properties of this enzyme. MDH has a mol. wt 60,000 and has two isoenzymes (pI 7.1 and 8.5), which are antigenically different. Isoenzyme (pI 7.1) is the antigen called 4, specific of the genus<em>Schistosoma</em> and is highly immunogenic. By immunofluorescence, MDH was localized in the cellular layer of the coecum. Mice immunized with purified MDH exhibited a significant decrease of worm burden.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 9","pages":"Pages 633-638"},"PeriodicalIF":0.0000,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90036-6","citationCount":"16","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078900366","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 16
Abstract
Affinity chromatography with AMP-Sepharose allows us to purify malate dehydrogenase ofSchistosoma mansoni and to perform the studies about the immunochemical and biological properties of this enzyme. MDH has a mol. wt 60,000 and has two isoenzymes (pI 7.1 and 8.5), which are antigenically different. Isoenzyme (pI 7.1) is the antigen called 4, specific of the genusSchistosoma and is highly immunogenic. By immunofluorescence, MDH was localized in the cellular layer of the coecum. Mice immunized with purified MDH exhibited a significant decrease of worm burden.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
