3D-reconstruction of synapses based on EM images

Abstract

3D reconstruction from the microscopy images of serial sections plays an important role in analyzing structure of biological specimens, such as neuronal circuits in brain tissue. This paper is focusing on the 3D reconstruction of synapses which is a branch of micro reconstruction of brain. Having analysed the structure of synapses, we first detect and locate them in serial sections with cascade AdaBoost algorithm, then optimize the shape of synapses by constructing suitable fitting functions and segment them with morphology processing method. Taking the connection of adjacent sections and synaptic extensibility in space into consideration, we complete the reconstruction of synapses which is based on the segmentation in serial sections. Actually all our works are based on the registration of serial sections.