Quantitative Evaluation of Micro-motion of Vascular Endothelial Cells in Electrical Cell-substrate Impedance Sensing (ECIS) Method Using a Precision Mathematical Model

N. Goda, Y. Yamamoto, T. Nakamura, T. Kusuhara, S. Mohri, N. Kataoka, F. Kajiya
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引用次数: 3

Abstract

We constructed a precision mathematical model for quantitative evaluation of the micro-dynamics of cultured cells measured with ECIS (electrical cell-substrate impedance sensing) system that could separately estimate cell-to-cell distance and cell-to-substrate distance. For wide applications of this method, we constructed mathematical models describing for some kinds of cell and some kinds of confluent conditions. The mathematical models of these impedances could be fit well especially from 1 kHz to 10 kHz, which were most interesting frequency range for micro-dynamic analysis in ECIS methods
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用精确数学模型定量评价电-基质阻抗传感(ECIS)方法中血管内皮细胞微运动
我们构建了一个精确的数学模型,用于用ECIS(电细胞-底物阻抗传感)系统测量培养细胞的微动力学,可以分别估计细胞与细胞之间的距离和细胞与底物之间的距离。为了使该方法得到广泛的应用,我们建立了描述几种单元和几种汇合条件的数学模型。这些阻抗的数学模型可以很好地拟合,特别是在1 kHz到10 kHz范围内,这是ECIS方法中最感兴趣的微动力分析频率范围
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