{"title":"Supplementary Information Text","authors":"Xiaolei Wu, Yuntian Zhu","doi":"10.1201/9781003153078-37","DOIUrl":null,"url":null,"abstract":"In Vitro Callus Production. Induction of in vitro callus production was performed as previously described (Iwase et al., 2011). Etiolated hypocotyls were excised from mutant and wild type seedlings 4 days after germination. Mutant and wild type explants were cultured for 30 days side-by-side on MS medium supplemented with Gamborg’s vitamins, 2% glucose, 0.05% 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.8 with KOH, 0.8% phytoagar, and the indicated concentrations of NAA and kinetin.","PeriodicalId":106290,"journal":{"name":"Heterostructured Materials","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Heterostructured Materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1201/9781003153078-37","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
In Vitro Callus Production. Induction of in vitro callus production was performed as previously described (Iwase et al., 2011). Etiolated hypocotyls were excised from mutant and wild type seedlings 4 days after germination. Mutant and wild type explants were cultured for 30 days side-by-side on MS medium supplemented with Gamborg’s vitamins, 2% glucose, 0.05% 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.8 with KOH, 0.8% phytoagar, and the indicated concentrations of NAA and kinetin.