S. Hamada, Kazuo Nakajima, C. Namiki, T. Serikawa, M. Hayashi
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引用次数: 8
Abstract
The micronucleus assay was conducted with 7 chemicals (2-acetylaminofluoren [2-AAF], 1-β-D-arabinofuranosylcytosine [Ara-C], colchicine, cyclophosphamide [CP], methyl methanesulfonate [MMS], potassium bromate [KBrO3], urethane) in male and female rats to determine whether the results varied with sex. Each chemical was administered twice orally, 24 h apart, to 5 rats in each of 3 dosage groups and collected bone marrow and peripheral blood 24 h later. Sex differences were observed in micronucleus induction in both polychromatic erythrocytes (bone marrow) and reticulocytes (peripheral blood), which we attributed to a sex difference in hematopoiesis. In spite of those differences, both sexes showed positive responses. We concluded that the rat is suitable for the micronucleus assay regardless of sex.
采用2-乙酰氨基氟[2-AAF]、1-β- d -阿拉伯糖胞嘧啶[Ara-C]、秋水仙碱、环磷酰胺[CP]、甲磺酸甲酯[MMS]、溴酸钾[KBrO3]、氨基甲酸乙酯]等7种化学物质对雌雄大鼠进行微核测定，以确定结果是否存在性别差异。3个剂量组各5只大鼠，每组24 h口服2次，24 h后采集骨髓和外周血。在多染红细胞(骨髓)和网织红细胞(外周血)的微核诱导中观察到性别差异，我们将其归因于造血的性别差异。尽管存在这些差异，但两性都表现出积极的反应。我们的结论是，无论性别，大鼠都适合进行微核试验。
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