Studies on the procedure of mutation breeding with, six-rowed barley. : I. The methods of procedure in X1 and X2 generations.

G. Takeda, Masujiro Kan
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Abstract

The methods of mutation breeding were applied on six rowved barly in three ways. The dormant seeds were treated with 15 Kr of X-rays. Method A : After 3 ears were havested from each X1 Plant and one grain was removed from each ear, these grains were planted in bulk at X2 Mlethod B : After one ear was harvested from each Xl plant, the grains were planted separately as ear progenies at X2Method C : After 3 ears were harvested from each Xi Plant, the grains were planted separately as plant progenies at X2 Method A was compared with B and C. The eharacters mainly analyzed were earliness and stems;shortness. The selection of such aberrants was carried out in X2 (Table 1), and these were confirmed as mutants in X3 (Tables 2 and 3) . In the case of method A-ll (Table 1), selection was made by measuring stem length at X2 generation. In the case of other methods, selection was made by observation. 1) By using method A, the mutant types found n X2 Were more frequently observed compared with the other two methods and the mutant much deviated from the parent type were involved.This result was compatible with the theoretical consideration of YOSHIDA (1962). 2) Much better selection of short stem mutants was made in X2 by method A (I+II) than by methods B and C. This result demonstrates that the individual selection using this measurement was made much better than the pedigree selectian through observation.
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六棱大麦诱变育种程序的研究。1 . X1、X2代的程序方法。
采用三种方法对6株大麦进行了诱变育种。休眠种子用15kr的x射线处理。方法一:3耳后被从每个X1 havested植物和一粒从每只耳朵,这些散装谷物种植X2 Mlethod B:一只耳朵从每个Xl植物收获后,分别种植谷物在X2Method耳朵两个C:从每个ξ3耳朵后收获植物,分别种植谷物作为植物后代X2方法A与B和C . eharacters主要分析了早熟和茎,呼吸急促。在X2中对这些异常进行筛选(表1),在X3中确认为突变体(表2和表3)。在a - all方法中(表1),通过测量X2代的茎长进行选择。在其他方法的情况下,选择是通过观察。1)与其他两种方法相比,A方法在n X2中发现的突变型更频繁,并且涉及与亲本型偏差较大的突变体。这一结果与吉田(1962)的理论考虑是一致的。2)方法A (I+II)对X2短茎突变体的选择效果明显优于方法B和方法c,说明该方法的个体选择效果明显优于观察的家系选择。
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