The Effect of Ethyl Acetate Fraction of Bangle (Zingiber Cassumunar Roxb.) Rhizome Extracts on Interleukin-10 and Interleukin-14 Expression in Vitro

Abstract

An unbalanced immune system results in reduced ability to protect the body from pathogens. Immune system imbalances can be restored with immunomodulators. One plant that has the potential to be immunomodulatory is the bangle rhizome (Zingiber cassumunar Roxb.) Which has curcumin as an immunomodulator. Its activity as an immunomodulator can be seen by increasing the expression of IL-10 and IL-14 which play an important role in controlling the immune response. This study aims to determine the effect of ethyl acetate fraction of bangle extract (Zingiber cassumunar Roxb.) on the expression of IL-10 and IL-14 in vitro. Macrophages were isolated from the peritoneum of male mice Balb / C strain, then cultured for 24 hours. The macrophages were treated with ethyl acetate fraction of bangle extract with concentrations of 25, 50, 100 μg/mL and the control group did not treated with ethyl acetate fraction of bangle extract. The method used to analyze the expression of IL10 and IL-14 is immunocytochemistry. Examination of the presence of curcumin is carried out by Thin Layer Chromatography (TLC) method. The data obtained were analyzed using a one-way ANOVA statistical test with a confidence level of 95%. The results showed that the administration of ethyl acetate fraction of bangle extract in mice macrophages with a concentration of 25, 50, 100 μg/mL compared to the negative control group had a significant difference (p <0.05) in IL-10 and IL-14 expression. In IL-10 there was an increase in percentage of expression in the control group, 25, 50, 100 μg/mL, respectively 55.83, 62.047, 66.593, and 74.387%. In the control group and 25, 50, and 100 μg/mL had significant differences between groups. In IL-14 there was an increase in the percentage of expression in the control group, 25, 50, 100 μg/mL, respectively 48.017, 70.649, 78.821, and 80.645%. In groups with a concentration of 50 μg/mL with 100 μg/mL did not show a significant difference with a significance value of 0.407. In conclusion, the administration of ethyl acetate fraction of bangle extract with a concentration of 25, 50, 100 μg/mL can be used as an immunomodulator by increased expression of IL-