Current Approaches in Immunoassay Methods Focus on Skeletal Muscle Proteins

G. Gaina
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Abstract

The skeletal muscle is a complex tissue that represents most of the muscle tissue in mammals and plays a key role in health and in the body’s function. It is a heterogeneous tissue whose contractile and metabolic functions depend on type, size, and quality of a large number of proteins. The multitude of proteins, the relationships that exist between them, and functional changes that occur in different muscle pathologies make their investigation to be challenged. In this chapter, current approaches in proteomic studies, its application, specific technical advice, and recent progress of the most important techniques based on antigen-antibody interactions used for the analysis of muscle proteins involved in different muscle diseases are presented.
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目前的免疫分析方法主要集中在骨骼肌蛋白上
骨骼肌是一种复杂的组织,代表了哺乳动物的大部分肌肉组织,在健康和身体功能中起着关键作用。它是一种异质组织,其收缩和代谢功能取决于大量蛋白质的类型、大小和质量。大量的蛋白质,它们之间存在的关系,以及在不同肌肉病理中发生的功能变化,使他们的研究受到挑战。在这一章中,介绍了蛋白质组学研究的当前方法,其应用,具体的技术建议,以及基于抗原-抗体相互作用的最重要技术的最新进展,这些技术用于分析与不同肌肉疾病有关的肌肉蛋白质。
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