{"title":"[The mechanism of action of low doses of gamma radiation connected with the development of human cell resistance to mutagens].","authors":"T A Sinel'shchikova, G D Zasukhina","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The human fibroblasts were gamma-irradiated with low doses (0.07-0.21 Gy). After a short time interval (3 h), a study was made of the postirradiation viability of cells (by the trypan blue dye exclusion method); post-N-methyl-N'-nitro-nitrosoguanidine-DNA synthesis (by 3H-thymidine incorporation immediately after the mutagen treatment) and postirradiation induction of DNA single-strand breaks (by alkaline elution of cells lysed on the membrane filters). The preirradiation of cells with low doses of gamma-rays was shown to render the cells resistance to induction of DNA breaks by the following exposure to gamma-radiation. The survival rate increased; DNA synthesis was resistant to alkylation damage in these cells, as compared to nonirradiated cells.</p>","PeriodicalId":20808,"journal":{"name":"Radiobiologiia","volume":"32 6","pages":"826-9"},"PeriodicalIF":0.0000,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiobiologiia","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The human fibroblasts were gamma-irradiated with low doses (0.07-0.21 Gy). After a short time interval (3 h), a study was made of the postirradiation viability of cells (by the trypan blue dye exclusion method); post-N-methyl-N'-nitro-nitrosoguanidine-DNA synthesis (by 3H-thymidine incorporation immediately after the mutagen treatment) and postirradiation induction of DNA single-strand breaks (by alkaline elution of cells lysed on the membrane filters). The preirradiation of cells with low doses of gamma-rays was shown to render the cells resistance to induction of DNA breaks by the following exposure to gamma-radiation. The survival rate increased; DNA synthesis was resistant to alkylation damage in these cells, as compared to nonirradiated cells.