Uji Aktivitas Antioksidan Ekstrak Metanol Daun Ramania (Bouea macrophylla Griffith.) Menggunakan Metode DPPH

Dimas Satya Aiyuba, Aditya Noviadi Rakhmatullah, Ratna Restapaty
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Abstract

Humans have a body that is equipped with a defense system that is considered to be an antioxidant which can minimize the damage caused by free radicals. Ramania (Bouea macrophylla Griffith.) is an herbal plant that has antioxidant properties and inhibits free radicals. This study aims to determine what are the active compounds contained in ramania leaves and determine the IC50 value. Maceration extraction method, obtained the yield of extract 42.5699 g, and the content of compounds contained in the extract are flavonoid compounds, saponins, tannins, steroids, and alkaloids. The extract was tested for antioxidant activity using the DPPH radical inhibition method quantitatively using a UV-Vis spectrophotometer with quercetin as a positive control. In testing the antioxidant quercetin with concentrations of 1, 2, 3, 4, 5 ppm, the results were 3.3434 ppm. In the antioxidant test of methanol extract with concentrations of 2, 4, 6, 8, 10 ppm, the results were 6305 ppm. The results obtained IC50 value of 6.305 ppm which is included in the category of very strong antioxidants. It can be concluded that the methanol extract of Ramania leaves has antioxidant activity.
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抗氧化物提取物的活性试验Ramania (Bouea macrophylla Griffith)。使用DPPH方法
人体有一种防御系统,被认为是一种抗氧化剂,可以最大限度地减少自由基造成的损害。拉曼尼亚(Bouea macrophylla Griffith.)是一种具有抗氧化性能和抑制自由基的草本植物。本研究的目的是确定拉曼尼亚叶片中含有哪些有效成分,并确定其IC50值。浸渍提取法,得到的提取物得率为42.5699 g,提取物中所含化合物有黄酮类化合物、皂苷类化合物、单宁类化合物、甾体类化合物和生物碱类化合物。以槲皮素为阳性对照物,采用紫外-可见分光光度计,采用DPPH自由基抑制法定量测定提取物的抗氧化活性。抗氧化剂槲皮素在浓度为1、2、3、4、5 ppm时的测试结果为3.3434 ppm。在浓度为2、4、6、8、10 ppm的甲醇提取物抗氧化试验中,结果为6305 ppm。所得IC50值为6.305 ppm,属于强抗氧化剂范畴。由此可见,拉马尼亚叶甲醇提取物具有抗氧化活性。
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