Expression of apelin is related to oxidative damage in heart tissue of rats during chronic systemic hypoxia

H R Helmi, F. Ferdinal, A. Prijanti, S. A. Jusman, Frans D Suyatna
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引用次数: 1

Abstract

Background: Chronic systemic hypoxia is severe environmental stress for the heart and might lead to the development of heart failure. Apelin is an endogenous peptide that has been shown to have various beneficial effects on cardiac function. Apelin appears to have a role to play in the ventricular dysfunction and maintaining the performance of the heart. Objectives: In the present study we want to investigate the adaptive response of heart tissue to chronic systemic hypoxia and the correlation with apelin expression and oxidative stress in rat.  Methods: An experimental study was performed using 28 Sprague-Dawley male rats, 8 weeks of age. Rats were divided into 7 groups 4 each, namely control group; normoxia (O2 atmosphere) and the treatment group of hypoxia (8% O2) for 6 hours; 1;3;5;7 and 14 days respectively. Body weight and heart weight were measured at each treatment. Ventricular thickness was measured by caliper, Apelin mRNA was measured using real-time qRT-PCR with Livak formula and malondialdehyde (MDA) level was used to assess oxidative stress due to cardiac tissue hypoxia. Results: Macroscopic exams showed hypertrophy at day 7th. The relative expression of Apelin mRNA in hypoxic heart is decreased at the beginning and then increased, starting from day-7 to day-14. The MDA levels were significantly increased from day-7 and were strongly correlated with relative expression Apelin. Conclusion:  It is concluded that the increase of Apelin expression is related to oxidative stress in heart tissue of rats during chronic systemic hypoxia.
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慢性全身性缺氧大鼠心脏组织中apelin的表达与氧化损伤有关
背景:慢性全身性缺氧是心脏的严重环境应激,可能导致心力衰竭的发展。Apelin是一种内源性肽,已被证明对心功能有各种有益作用。Apelin似乎在室性功能障碍和维持心脏功能方面发挥作用。目的:探讨大鼠心脏组织对慢性全身性缺氧的适应性反应及其与apelin表达和氧化应激的关系。方法:采用28只8周龄雄性Sprague-Dawley大鼠进行实验研究。将大鼠分为7组,每组4只,即对照组;常氧(O2气氛)和缺氧(8% O2)治疗组6 h;第1、3、5、7、14天。在每次治疗时测量体重和心脏重量。用卡尺测量心室厚度,用Livak式实时定量rt - pcr检测Apelin mRNA,用丙二醛(MDA)水平评估心脏组织缺氧引起的氧化应激。结果:肉眼检查示第7天肥厚。Apelin mRNA在缺氧心脏中的相对表达先降低后升高,从第7天开始到第14天结束。MDA水平从第7天开始显著升高,且与Apelin的相对表达量密切相关。结论:慢性全身性缺氧大鼠心脏组织中Apelin表达升高与氧化应激有关。
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