H Koziel, D J Williams, M Y Armstrong, F F Richards, J A Fishman, R A Ezekowitz, A Warner, J Fuglestad, R M Rose
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Abstract
Understanding the pathophysiology of Pneumocystis carinii infection has been limited by the availability of methods for precisely measuring the interaction of P. carinii with host cells. Here we describe a new method which allows for the rapid assessment of P. carinii binding to, and internalization by, adherent alveolar macrophages. The method is based on the detection of fluorescein-labelled P. carinii by an automated fluorescence measurement system.
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