Antibacterial Activity Analysis of Zanthoxylum Acanthopodium DC Extract on Bacteria of Bacillus Subtilis, and Salmonella Typhi

Proceedings of the Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia Pub Date : 1900-01-01 DOI:10.4108/eai.18-10-2018.2287303

N. Sihombing, Friska Sihombing, T. Juwitaningsih, D. Roza, D. Pasaribu

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Abstract

Research has been conducted on the antibacterial activity analysis of Zanthoxylum acanthopodium DC extract on Bacillus subtilis, Salmonella typhi using disc diffusion method. This study aims to determine the ability of Zanthoxylum acanthopodium DC extract in inhibiting the growth of Bacillus subtillis, and Salmonella typhi. Zanthoxylum acanthopodium DC was extracted by maceration method using 96% ethanol. In this extraction process, phytochemical test was carried out to find out the secondary metabolite content in Zanthoxylum acanthopodium DC. Phytochemical test indicate Zanthoxylum acanthopodium DC contained alkaloids, tannins, saponins, and steroids. Antibacterial activity analysis were measured in three concentrations of 25%, 50%, 75%. The best antibacterial activity was observed on Salmonella typhi bacteria with 75% extract concentration which produced a clear zone diameter of 19 mm and 20 mm.

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棘花椒提取物对枯草芽孢杆菌和伤寒沙门氏菌的抑菌活性分析

采用圆盘扩散法对棘花椒提取物对枯草芽孢杆菌、伤寒沙门氏菌的抑菌活性进行了研究。本研究旨在研究棘花椒提取物对枯草芽孢杆菌和伤寒沙门氏菌生长的抑制作用。采用96%乙醇浸渍法提取棘花椒。在提取过程中进行植物化学试验，测定棘花椒次生代谢物的含量。植物化学试验表明棘花椒含有生物碱、单宁、皂苷和类固醇。在25%、50%、75%浓度下进行抑菌活性分析。对伤寒沙门菌的抑菌效果最好，提取液浓度为75%，清带直径分别为19 mm和20 mm。

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Proceedings of the Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia

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