Detection of cytokines in skeletal muscle tissue using optical SPR sensing platform

Víctor Parra-Monreal, María A. Ortega-Machuca, J. Ramón‐Azcón, W. Svendsen, A. Romano-Rodríguez, Mauricio Moreno-Sereno
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Abstract

In this work we have explored the use of a Surface Plasmon resonance (SPR) phenomenon for the detection of interleukin-6 (IL-6), a pro-inflammatory cytokine. It plays an important role in the muscle tissues, having direct relation with muscle contraction and, thus, it is considered a biomarker for some types of muscular dystrophies. Here we show that SPR can be used as a real-time monitoring of the shift of the reflectance dip of a gold diffraction grating in front to the antibody adhesion to gold.
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利用光学SPR传感平台检测骨骼肌组织细胞因子
在这项工作中,我们探索了使用表面等离子体共振(SPR)现象来检测白细胞介素-6 (IL-6),一种促炎细胞因子。它在肌肉组织中起着重要作用,与肌肉收缩有直接关系,因此,它被认为是某些类型肌肉萎缩症的生物标志物。我们发现SPR可以用来实时监测前方金衍射光栅的反射倾角对抗体粘附金的变化。
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