Peningkatan Kadar Protein Terlarut pada Larutan Tepung Jagung Menggunakan Saccharomyces cerevisiae

S. Tridesianti
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Abstract

Poultry livestock are generally given feed in the form of corn kernels where the corn kernels contain high carbohydrates but have deficiencies of several amino acids. Amino acid deficiency can be overcome by increasing the protein content in the corn kernels. One way to increase the protein content in corn kernels is to carry out a fermentation process in corn flour solution. Therefore, this study aims to determine the effect of corn starch solution to increase dissolved protein levels in Saccharomyces cerevisiae biomass through a fermentation process. This study used a completely randomized design consisting of 8 treatments and 3 replications. The treatment volumes of corn flour solution were 0 mL, 5 mL, 10 mL, 15 mL, 20 mL, 25 mL, 30 mL and 35 mL. Data analysis used the calculation of variance and the BJND test. The results showed that the highest average dissolved protein content and total cell biomass of S. cerevisiae in the 15 ml treatment were 13.52%/g and 15.63 x 107 CFU/mL, the lowest average was in the 35 ml corn flour solution treatment, namely 0. 90%/gr and 3.01 x 107 CFU/mL. Based on the research, it can be concluded that corn flour solution can increase the dissolved protein content in S. cerevisiae biomass up to volume 15 mL/2 mL fermenter through the fermentation process. Based on the results of this study, it can be used as a basis for considering the use of S. cerevisiae fermenters to increase the nutritional value of corn seed-based animal feed.
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家禽家畜通常以玉米粒的形式喂养,玉米粒含有高碳水化合物,但缺乏几种氨基酸。氨基酸缺乏可以通过增加玉米粒中的蛋白质含量来克服。提高玉米粒中蛋白质含量的一种方法是在玉米粉溶液中进行发酵。因此,本研究旨在通过发酵过程确定玉米淀粉溶液对提高酿酒酵母生物量中溶解蛋白水平的影响。本研究采用完全随机设计,包括8个处理和3个重复。玉米粉溶液处理量分别为0 mL、5 mL、10 mL、15 mL、20 mL、25 mL、30 mL和35 mL,数据分析采用方差计算和BJND检验。结果表明,15 ml处理下酿酒酵母的平均溶解蛋白含量和细胞总生物量最高,分别为13.52%/g和15.63 × 107 CFU/ ml, 35 ml玉米粉溶液处理的平均值最低,为0。90%/gr, 3.01 × 107 CFU/mL。综上所述,在发酵过程中,玉米粉溶液可使酿酒酵母生物量中溶解蛋白含量提高至15 mL/2 mL。本研究结果可作为考虑利用酿酒酵母发酵罐提高玉米种子基动物饲料营养价值的依据。
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