C. Ortíz-de-Solórzano, T. Pengo, Miguel Galarraga, A. Muñoz-Barrutia
{"title":"Automation of the detection of lung cancer cells in minimal samples of bronchioalveolar lavage","authors":"C. Ortíz-de-Solórzano, T. Pengo, Miguel Galarraga, A. Muñoz-Barrutia","doi":"10.1109/ISBI.2008.4540995","DOIUrl":null,"url":null,"abstract":"We present the hardware and software specification of a quantitative, multidimensional and multispectral microscopy system designed for the detection of lung cancer using minimal samples of bronchoalveolar lavage (BAL). BAL samples were stained using FICTION: Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Neoplasms. Our system allows preliminary immunophenotypic detection of rare cancerous candidate cells, followed by accurate three-dimensional analysis of genomic integrity, to confirm or refute the initial assessment. Our results show that our automated analysis can accurately assist a human expert in the diagnostic evaluation of BAL samples.","PeriodicalId":184204,"journal":{"name":"2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro","volume":"123 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2008-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ISBI.2008.4540995","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
We present the hardware and software specification of a quantitative, multidimensional and multispectral microscopy system designed for the detection of lung cancer using minimal samples of bronchoalveolar lavage (BAL). BAL samples were stained using FICTION: Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Neoplasms. Our system allows preliminary immunophenotypic detection of rare cancerous candidate cells, followed by accurate three-dimensional analysis of genomic integrity, to confirm or refute the initial assessment. Our results show that our automated analysis can accurately assist a human expert in the diagnostic evaluation of BAL samples.