The Effect of Various Concentration BAP (6-Benzyl Amino Purine) on Orchid Growth (Macodes petola (Blume) Lindl.) In-Vitro

Muchfa Eprilia Muchsin, A. Supriatna, A. Adawiyah, Adisty Virakawugi Darniwa
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Abstract

Macodes petola (Blume) Lindl. has an attractive of leaves veins motif with letters pattern, so it has a high economic value. Tissue culture technique is reported as the best way of plant propagation in short time. BAP (6-Benzyl Amino Purine) has a role in stimulating shoot growth with a certain concentration. The purpose of this study was to determine the effect of Various Concentration BAP (6-Benzyl Amino Purine) on Orchid Growth (M petola (Blume) Lindl.) in vitro. This study used a completely randomized design (CRD) with 4 concentration BAP levels (0; 0.5; 1; 1.5 ppm) with 3 replication. Based on the results of the study, it showed that up to 60 DAP (Day After Planting) the composition of the media with a concentration of 0 ppm BAP had a higher average value for the increase in the stem height, number of shoots and number of roots, namely 1.77±0.798 cm, 2.4 ± 1,528 shoot and 2.4 ± 2,082 root. In contrast to the concentration of 1.5 ppm at this concentration it has a low average value the parameters of stem height, number of shoots and number of roots.
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不同浓度BAP(6-苄基氨基嘌呤)对兰科植物生长的影响体外
Macodes petola (Blume) Lindl。具有引人注目的叶脉图案和字母图案,具有很高的经济价值。组织培养技术是植物短期繁殖的最佳方法。6-苄基氨基嘌呤(BAP)在一定浓度下具有刺激茎部生长的作用。本研究旨在研究不同浓度的6-苄基氨基嘌呤(BAP)对兰花(M petola (Blume) Lindl.)体外生长的影响。本研究采用完全随机设计(CRD), 4种浓度BAP水平(0;0.5;1;1.5 ppm), 3次复制。研究结果表明,当BAP浓度为0 ppm的培养基组成达到60 DAP时,茎高、芽数和根数增加的平均值更高,分别为1.77±0.798 cm、2.4±1528个芽和2.4±2082个根。与1.5 ppm浓度相比,该浓度下的茎高、芽数和根数的平均值较低。
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