{"title":"yozG is needed for swarming in the undomesticated Bacillus subtilis strain NCIB 3610","authors":"Erin I. Hayes, J. E. Patrick","doi":"10.30956/mas-50","DOIUrl":null,"url":null,"abstract":"\n Bacillus subtilis is capable of swarming motility on semisolid surfaces. Here we characterize the swarming phenotype of a mutant in the gene of unknown function, yozG in the undomesticated strain 3610. The yozG mutant was unable to swarm. Swarming could be restored to the mutant by overexpression of the swarming regulator gene swrA or by overexpression of the flagella and chemotaxis operon. In addition, we were able to isolate two genetic suppressors of the yozG mutant that could also restore swarming. yozG is necessary for swarming in B. subtilis and likely acts upstream of the swarming regulator, SwrA.","PeriodicalId":443111,"journal":{"name":"Transactions of the Missouri Academy of Science","volume":"12 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transactions of the Missouri Academy of Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.30956/mas-50","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Bacillus subtilis is capable of swarming motility on semisolid surfaces. Here we characterize the swarming phenotype of a mutant in the gene of unknown function, yozG in the undomesticated strain 3610. The yozG mutant was unable to swarm. Swarming could be restored to the mutant by overexpression of the swarming regulator gene swrA or by overexpression of the flagella and chemotaxis operon. In addition, we were able to isolate two genetic suppressors of the yozG mutant that could also restore swarming. yozG is necessary for swarming in B. subtilis and likely acts upstream of the swarming regulator, SwrA.
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