Metil Paration’nun Piruvat Kinaz Enzim Aktivitesine Etkisi

Abstract

Enzyme Activity ABSTRACT Methyl parathion is an organophosphate group insecticide used against pests in agriculture and general fields. Insecticides have a significant toxic effect on the environment and living organisms. Methyl parathion, which causes different toxicity in humans, is also known to affect important enzymes that control metabolism. Pyruvate kinase that allosteric properties (EC.2.7.1.40) is the last enzyme of glycolysis and provides control in glycolysis followed by molecular pathways. In our study, the effect of Methyl parathion on Pyruvate kinase enzyme activity was investigated. Methyl parathion LD 50 dose (7mg.kg-1) was administered intraperitoneally to male and female rats at different periods (2, 4, 8, 16, 32, 64 and 72 hours). Pyruvate kinase enzyme activity was analyzed in liver, kidney, brain and small intestine tissues of rats. As a result of exposure of Methyl parathion, increases in Pyruvate kinase enzyme activity were generally determined in rat tissues. It was remarkable that the activations took place earlier, especially in the brain tissue. It was observed that these activations in liver, kidney and small intestine tissues were earlier in females than in males. As a result, the application of Methyl parathion was found to cause increases in pyruvate kinase enzyme activity in rat tissues. It was determined that methyl parathion affects glucose metabolism and the other metabolic pathways. Therefore, it should be considered that methyl parathion insecticide affects metabolism and may cause different health problems and precautions should be taken in use by paying attention to the application dose recommended by the World