3D nanoscope analyses on the retinas of mouse (Mus musculus)

I. Orujalipoor, A. Yoldaş, S. İde, C. Chiao, Yen-Chih Huang, U. Jeng
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Abstract

The retina segments obtained from Mice-664 C57BL/6J eyeballs were investigated at 23A1 IASW- Beamline, NSRRC. Transmission small and wide-angle scattering (SAXS and WAXS) measurements and the related data were used to reach nanoscale natural morphology and internal structural information of ROS. Rhodopsin macromolecules in their natural medium (inside of the rods and retina) were successfully detected. The X-ray scattering effects of rhodopsin macromolecules in mice eyeball and surgically separated retina samples were carried out to reach the most natural situation of the molecules in the same animal species and to determine detection limits of rhodopsin macromolecules. The quantitative results about the structures of ROS membranes, rhodopsins and α-helices trans membranes were also carried out in nanoscopic scale.
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小鼠视网膜的三维纳米显微镜分析
小鼠-664 C57BL/6J眼球的视网膜片段在NSRRC 23A1 IASW- Beamline上进行了研究。利用透射小广角散射(SAXS和WAXS)测量及相关数据,获得纳米尺度下活性氧的自然形态和内部结构信息。在其自然介质(视杆细胞和视网膜内)中成功检测到视紫红质大分子。研究视紫红质大分子在小鼠眼球和手术分离视网膜样品中的x射线散射效应,以达到分子在同一动物物种中的最自然状态,确定视紫红质大分子的检出限。在纳米尺度上对活性氧膜、视紫红质和α-螺旋反式膜的结构进行了定量分析。
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