PRECURSOR-INOCULUM INTERACTION FOR THE GROWTH PROMOTION OF COTTON UNDER FIELD CONDITIONS

Khalid Mahmood, Shahid Nazir, Muhammad Amjad Qureshi, Sajid ur Rahman, Muhammad Asif Ali
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Abstract

The present experiment was conducted during 2019-2021 at Agricultural Biotechnology Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan. The yield of local BT cotton variety FH-142 was evaluated using different combinations of rhizobacteria (free living diazotroph and phosphate solubilizer) with auxin precursor L-tryptophan (L-TRP). The experiment was comprised of seven treatments under Randomized Complete Block Design (RCBD) replicated thrice. The experiment was repeated for consecutive three years to find the effect of microbial inoculants with auxin precursor L-TRP and mean of three years was reported in the manuscript. The microbial inoculants were applied as seed coating. The bacterial inoculation showed promising results as compared to control. However, the consortium of PGPR and P-solubilizer with precursor had the significant effects on cotton yield during all three years of experiment. The consortium increased the yield parameters of cotton i.e., 1810 kg/ha in comparison with un-inoculated control i.e., 1672 kg/ha. Individual applications of PGPR and P-solubilizer also had significant effect on the yield compared to control. Hence, it was concluded that the interaction of bacterial consortium and precursor (precursor inoculum interaction) proved to be the best to improve the cotton yield.
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田间条件下前体-接种剂互作促进棉花生长的研究
本试验于2019-2021年在巴基斯坦费萨拉巴德Ayub农业研究所农业生物技术研究所进行。采用根瘤菌(游离重氮营养菌和磷酸盐增溶剂)与生长素前体l -色氨酸(L-TRP)的不同组合,对当地BT棉品种FH-142的产量进行了评价。试验采用随机完全区组设计(RCBD),共7个处理,重复3次。本实验连续三年重复进行,以发现微生物接种剂对生长素前体L-TRP的影响,文中平均三年报道。采用微生物接种剂作为种子包衣。与对照相比,细菌接种显示出良好的结果。但在3年的试验中,PGPR与p -增溶剂及前驱体联合施用对棉花产量的影响均显著。与未接种对照(1672 kg/ha)相比,该组合可使棉花产量参数提高1810 kg/ha。与对照相比,单独施用PGPR和p增溶剂对产量也有显著影响。综上所述,细菌联合体与前体相互作用(前体接种物相互作用)是提高棉花产量的最佳途径。
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