Genetic Diversity 20 Bean Varieties using Microsatellite Technique (SSR)

Phuoc Trong Nguyen, Khang Minh Le, Hieu Bui Chi, Loan Hong Thi Nguyen, Lang thi Nguyen
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Abstract

Agro-morphological characters and PCR based markers have provided valuable information about genetic diversity of bean collection in HATRI . Analysis on SSR molecular markers: out of a total of 44 primers conducted genetic diversity studies, only 28 primers amplified the product on 20 bean varieties. Through the SSR marker data with 28 primers used, 20 varieties are classified into 4 main groups. In the subgroup of the SSR on 28 molecular markers are noted with 4 distinct groups. Molecular markers to be able to indirectly assess the presence or absence of selected genes thanks to markers without environmental influences. The diversity index analyzes according to the high SSR method (H = 0.384) while the diversity index of stick beans. The results presented here are the first steps towards a better understanding of varieties introduced from countries and may help guide future research into the crop.
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利用微卫星技术(SSR)分析20个豆类品种的遗传多样性
农业形态特征和基于PCR的标记为HATRI地区收集的豆类遗传多样性提供了有价值的信息。SSR分子标记分析:在进行遗传多样性研究的44条引物中,只有28条引物在20个豆类品种上扩增了产物。通过28条引物的SSR标记数据,将20个品种划分为4个主要类群。在28个分子标记的SSR亚群中,有4个不同的类群。由于不受环境影响,分子标记能够间接评估所选基因的存在或不存在。多样性指数采用高SSR方法进行分析(H = 0.384),而棒豆的多样性指数采用高SSR方法进行分析。这里提出的结果是朝着更好地了解从各国引进的品种迈出的第一步,可能有助于指导未来对这种作物的研究。
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