Alberto Diaspro, Paolo Bianchini, Fabio Callegari, Lisa Cuneo, Riccardo Marongiu, Aymeric Le Gratiet, Ali Mohebi, M. Scotto, Colin J. R. Sheppard
{"title":"Emerging Mueller matrix microscopy applications in biophysics and biomedicine","authors":"Alberto Diaspro, Paolo Bianchini, Fabio Callegari, Lisa Cuneo, Riccardo Marongiu, Aymeric Le Gratiet, Ali Mohebi, M. Scotto, Colin J. R. Sheppard","doi":"10.1007/s40766-023-00046-5","DOIUrl":null,"url":null,"abstract":"Abstract Polarized and wide-field light microscopy has been studied for many years to develop accurate and information-rich images within a focused framework on biophysics and biomedicine. Technological advances and conceptual understanding have recently led to significant results in terms of applications. Simultaneously, developments in label-free methods are opening a new window on molecular imaging at a low dose of illumination. The ability to encode and decode polarized light pixel by pixel, coupled with the computational strength provided by artificial intelligence, is the running perspective of label-free optical microscopy. More specifically, the information-rich content Mueller matrix microscopy through its 16 elements offers multimodal imaging, an original data set to be integrated with other advanced optical methods. This dilates the spectrum of possible and potential applications. Here, we explore the recent advances in basic and applied research towards technological applications tailored for specific questions in biophysics and biomedicine.","PeriodicalId":54452,"journal":{"name":"Rivista Del Nuovo Cimento","volume":"14 1","pages":"0"},"PeriodicalIF":5.9000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rivista Del Nuovo Cimento","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s40766-023-00046-5","RegionNum":2,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PHYSICS, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract Polarized and wide-field light microscopy has been studied for many years to develop accurate and information-rich images within a focused framework on biophysics and biomedicine. Technological advances and conceptual understanding have recently led to significant results in terms of applications. Simultaneously, developments in label-free methods are opening a new window on molecular imaging at a low dose of illumination. The ability to encode and decode polarized light pixel by pixel, coupled with the computational strength provided by artificial intelligence, is the running perspective of label-free optical microscopy. More specifically, the information-rich content Mueller matrix microscopy through its 16 elements offers multimodal imaging, an original data set to be integrated with other advanced optical methods. This dilates the spectrum of possible and potential applications. Here, we explore the recent advances in basic and applied research towards technological applications tailored for specific questions in biophysics and biomedicine.
期刊介绍:
La Rivista del Nuovo Cimento is an international peer-reviewed journal. It publishes monographs in all fields of physics. These monographies aim at presenting the state of the art of topical subjects of relevant interest for the community. Usually, authors are invited and topics suggested by the Deputy Editors-in-Chief, but also spontaneous submissions are examined.