Cultivation of Saprobic Basidiomycetes (Bjerkandera adusta, Phlebiopsis gigantea and Sistotrema brinkmannii) Using Different Biological Waste Substrates

Natālija Burņeviča, Dārta Kļaviņa, Laima Ciseļonoka, Dainis E. Runģis, Tālis Gaitnieks
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Abstract

Abstract Development of new biological preparations to control Heterobasidion root rot is a complex process, but when a potential antagonist is identified, cultivation of the fungus is required. In this study, five different substrates (deciduous sawdust, coniferous sawdust, rye bran, straw and corn kernels) were tested as substrates for the cultivation of three fungal species: Bjerkandera adusta, Phlebiopsis gigantea , and Sistotrema brinkmannii , which could be potentially used against Heterobasidion spp. Mycelial growth was evaluated visually, and oidia production was estimated microscopically. In the straw substrate, P. gigantea produced significantly more ( p < 0.05) oidia compared to the other substrates. In addition, oidia production at two different incubation temperatures were compared. As a result, the best substrate for cultivation of all three fungal species was coniferous sawdust.
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利用不同生物废弃物基质培养腐坏担子菌(Bjerkandera adusta, Phlebiopsis gigantea和Sistotrema brinkmannii
开发新的生物制剂来防治异黑霉根腐病是一个复杂的过程,但当发现潜在的拮抗剂时,就需要进行真菌的培养。本研究采用落叶木屑、针叶木屑、黑麦麸皮、秸秆和玉米粒等5种不同基质,分别培养adusta Bjerkandera、Phlebiopsis gigantea和Sistotrema brinkmannii 3种具有拮抗异黑霉潜力的真菌,通过目测和显微观察对菌丝生长进行了评价。在秸秆基质中,p . gigantea显著增加(p <0.05),与其他底物相比。此外,还比较了两种不同孵育温度下的卵泡产量。结果表明,三种真菌的最佳培养基质均为针叶木屑。
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来源期刊
CiteScore
0.70
自引率
0.00%
发文量
61
审稿时长
20 weeks
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