Evaluating the antimicrobial activity, antioxidant potential and GC-MS based chemical characterization of Cannabis sativa found in Pakistan

Q3 Medicine Herba Polonica Pub Date : 2023-09-30 DOI:10.5604/01.3001.0053.9615

ANAM AMIN SHAMI, TASBEEHA MARJAN, SYED ALI RAZA, HAMID MUKHTAR, MUHAMMAD WASEEM MUMTAZ, NEELAM CHAUDHARY, MUHAMMAD TAYYAB AKHTAR

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Abstract

Introduction: Cannabis is a dioecious plant belonging to the family Cannabaecae.Objective: The present work was conducted to study the antimicrobial activity, antioxidant potential andGC-MS-based metabolite profiling of Cannabis.Methods: The antimicrobial activity of Cannabis extract was assessed through disc-diffusion method. Theanti-oxidant potential of CEO was checked through DPPH assay and FRAP assay. We also assessed the totalphenolic (TPC) and flavonoid content (TFC) of the extract. The phytocompounds present in the Cannabisextract were characterized by using GC-MS.Results: The antimicrobial assay revealed significant activity against Pseudomonas, Staphylococcus aureusand Klebsiella pneumoniae, whereas moderate antioxidant activity was shown by Cannabis extract, having76.54% inhibition in DPPH assay and 56.10% inhibition in FRAP assay. TPC and TFC were 26.77 mg GAE/gD.E. and 34.45 mg QE/g D.W, respectively. The chemical characterization of Cannabis extract by GC-MSshows the presence of cannabinoids, terpenes, aliphatic compounds, alkanes and sesquiterpenes.

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评价巴基斯坦产大麻的抗菌活性、抗氧化潜力及GC-MS化学性质

简介:大麻是一种属于大麻科的雌雄异株植物。目的:研究大麻的抑菌活性、抗氧化潜力及gc - ms代谢物谱。方法:采用圆盘扩散法测定大麻提取物的抑菌活性。通过DPPH法和FRAP法检测CEO的抗氧化能力。我们还评估了提取物的总酚(TPC)和类黄酮(TFC)含量。采用气相色谱-质谱法对大麻提取物中的植物化合物进行了表征。结果:大麻提取物对假单胞菌、金黄色葡萄球菌和肺炎克雷伯菌的抑菌活性显著，对DPPH和FRAP的抑菌活性分别为76.54%和56.10%。TPC和TFC分别为26.77 mg GAE/ gde。分别为34.45 mg QE/g D.W。气相色谱-质谱分析表明，大麻提取物中含有大麻素、萜烯、脂肪族化合物、烷烃和倍半萜烯。

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