Study of the Effect of Palmitic Acid on the Expression of Myostatin mRNA and its Cytotoxic Properties in the Culture of Myoblast Cells and the Possibility of Exogenous Regulation

Q2 Pharmacology, Toxicology and Pharmaceutics Current Bioactive Compounds Pub Date : 2023-10-25 DOI:10.2174/0115734072273072231017104102

Vladimir G. Kukes, Vladimir A. Furalyov, Albina А. Gazdanova, Olga K. Parfenova, Dmitry V. Grishin, Nikita G. Sidorov

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Abstract

Objective: To study the cytotoxic effect of palmitic acid on myoblasts in vitro and the influence of this toxicant on the expression of myostatin mRNA in myoblast culture. Methods: To research the protective action against these processes of a compound with antioxidant activity, for which 2-ethyl-6-methyl-3-hydroxypyridine malate (ethoxidol) was chosen. Results: Our studies have shown that palmitic acid has a noticeable cytostatic effect on myoblasts in vitro, significantly suppressing their proliferation: the rate of MTT recovery in myoblasts treated with palmitate was only 9.6% of that rate in control myoblasts. In experiments, it was shown that palmitic acid slightly activated the expression of myostatin mRNA. At the same time, the protective effect of 2-ethyl-6-methyl-3-hydroxypyridine malate was not so pronounced. Conclusion: The results of our research indicate that the activation of myostatin synthesis is not one of the main causes of the development of myodystrophy in obese people or people following a high-lipid diet, while the direct cytotoxic effect of palmitic acid on myoblasts is. It is obvious that the use of antioxidants such as ethoxide has a protective effect on myoblasts in the experiment and may have a certain potential in clinical practice.

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棕榈酸对成肌细胞培养中肌生长抑制素mRNA表达及其细胞毒性的影响及外源调控可能性的研究

目的:研究棕榈酸对体外培养成肌细胞的细胞毒作用及对培养成肌细胞中肌生长抑制素mRNA表达的影响。方法:以2-乙基-6-甲基-3-羟吡啶苹果酸乙氧基醇为研究对象，研究具有抗氧化活性的化合物对上述过程的保护作用。结果:我们的研究表明，棕榈酸对体外培养的成肌细胞具有明显的细胞抑制作用，显著抑制成肌细胞的增殖:棕榈酸处理的成肌细胞MTT恢复率仅为对照成肌细胞的9.6%。实验表明，棕榈酸能轻微激活肌生长抑制素mRNA的表达。同时，2-乙基-6-甲基-3-苹果酸羟吡啶的保护作用不明显。结论:我们的研究结果表明，肌生长抑制素合成的激活不是肥胖或高脂饮食人群肌营养不良发生的主要原因之一，而棕榈酸对成肌细胞的直接细胞毒作用才是。实验结果表明，乙醇等抗氧化剂的使用对成肌细胞具有明显的保护作用，在临床应用中可能具有一定的潜力。

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来源期刊

Current Bioactive Compounds Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.90

自引率

0.00%

发文量

112

期刊介绍： The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.