Influence of Thidiazuron on in vitro regeneration potential of Kaempferia parviflora Wall. Ex Baker from Eastern India

Abstract

An in vitro regeneration system was standarized on a traditional medicinal plant of Kaempferia parviflora. The rhizome sprouted buds upon transfer to MS basal medium with kinetin (3mg/l) and naphthalene acetic acid (0.5mg/l) were found optimum exhibiting maximum percent of shoot initiation as 90.6 ± 0.2. Maximum number of shoots as 8.1 ± 0.3 and maximum number of roots as 22.0 ± 0.3 were found in benzyladenine (3mg/l), naphthalene acetic acid (1mg/l) and thidiazuron (0.5mg/l) combination. Then the micropropagated plants were transferred to field for successful establishment as 90%. Furthermore, the morphological characteristic analysis was done between the conventional and micropropagated plants which showed very less significant variation. Thus the present protocol reports the propagation of K. parviflora plantlets which could be used for commercial exploitation in future.