Effect of Platelet-rich Plasma Combined with Marburg Bone Bank-prepared Bone Graft in Rabbit Bone Defect Model

Abstract

Background: An effective technique for inducing bone formation without using an autograft has yet to be established. Platelet-rich plasma (PRP), which can be obtained easily from whole blood, contains substantial growth factors (GFs) that can facilitate bone regeneration and growth. Objectives: This study aimed to evaluate the effect of PRP combined with a Marburg bone bank-prepared bone graft in a rabbit bone defect model. Methods: This study utilized 32 rabbits (n = 16 in each group). Bone defects were intentionally made in the femur, and the bone allograft used was the human femoral head prepared according to the Marburg bone bank. Rabbits were divided into Marburg bone graft (MBG) and MBG+PRP groups. Histopathological and histomorphometric analyses were conducted 14 and 30 days post-surgery. Results: A greater new bone formation was detected in both groups on the 14th and 30th days (P = 0.001). Furthermore, more pronounced angiogenesis was found in the MBG+PRP group than in the MBG group (P = 0.001). Conclusions: The MBG-PRP complex significantly enhanced bone tissue repair in bone defects. The inclusion of PRP was found to promote angiogenesis and stimulate the formation of new bone tissue, further supporting the beneficial effects of this combination in the healing process.