Phytochemical Analysis, Antifungal and Antibacterial Screening of Aegle marmelos: A Guyana Floral Extract

Brij Bhushan Tewari, Subramanian Gomatinayagam
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Abstract

The plant material, leaves of Aegle marmelos were collected from Cove and John Ashram, Georgetown, Guyana. Leaves are dried in oven at 50-55 ºC for 72 h. The moisture content is calculated. The dried leaves were grounded and extracted in each acetone, ethanol and methanol solvents. Extracts were collected and evaporation of solvent was done on rotavapour. The antimicrobial and antifungal activity of leaf extract were examined by well diffusion, poison plate, paper disc plate and streak plate methods. In Aegle marmelos leaves extract studies, maximum and minimum antimicrobial potential was observed for methanol and acetone solvents, respectively. Antimicrobial potential of leave extract were also found to be maximum and minimum in Candida albicans and Escherichia coli, respectively in most assay studies. The phyto constituents, tannins, flavonoids, alkaloids, terpenoids, phenol, steroids and phytosteroids were found to present in methanol leave extract of Aegle marmelos. Present study is focus on phytochemical analysis, antifungal and antibacterial screening of Aegle marmelos leaves extracts.
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一种圭亚那花提取物的植物化学分析、抗真菌和抗菌筛选
植物材料,柑橘叶收集于Cove和John Ashram,乔治城,圭亚那。在50-55℃的烘箱中干燥72小时,计算水分含量。将干叶研磨,分别在丙酮、乙醇和甲醇溶剂中提取。收集提取液,用旋蒸器蒸发溶剂。采用孔扩散法、毒板法、纸盘板法和条纹板法检测其抑菌活性。在柑橘叶提取物的研究中,甲醇溶剂和丙酮溶剂的抑菌潜力最大,丙酮溶剂的抑菌潜力最小。在大多数试验研究中,叶提取物对白色念珠菌和大肠杆菌的抑菌潜力分别最大和最小。结果表明,甜瓜甲醇叶提取物中含有植物性成分、单宁、黄酮类、生物碱、萜类、酚类、甾体和植物甾体。本文对蜜瓜叶提取物进行了植物化学分析、抗真菌和抗菌筛选等方面的研究。
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