Comparative Study on Three Reagents for Rapid Biochemical Identification of Enterobacter sakazakii

Abstract

Objective: To compare the reliability of three biochemical identification reagents for Enterobacter sakazakii . Method: Based on the traditional biochemical identification tube in our laboratory, a dry state biochemical identification strip was prepared by referring to the biochemical reaction in GB 4789.40-2016. Using the traditional biochemical tube (JDG) as a reference, 50 strains of E. sa-kazakii , their close source bacteria, and isolated strains were tested to compare the identification effects of our laboratory’s self-made HKEasyID E . sakazakii biochemical identification strip (HKEa-syID), A company’s E . sakazakii biochemical identification strip (KitA), and B company’s E . sakaza-kii biochemical identification strip (KitB). Result: The identification results of 50 test strains showed that the average coincidence rates of HKEasyID, KitA and KitB with JDG traditional liquid identification kit were 98.67%, 88.44%, and 90.22%, respectively. HKEasyID had a higher coincidence rate with JDG compared to KitA and KitB; the average compliance rates of the four identification kits, HKHKEasyID, KitA, KitB, and JDG, compared to the standard requirements, were 97.11%, 87.11%, 89.11%, and 97.33%, respectively. HKEasyID and JDG were equivalent, higher than KitA and KitB. Conclusion: The HKEasyID identification strip is superior in operability and compliance to the identification strips of Company A and Company B, and is similar in reliability to traditional biochemical tubes. It can be used as a conventional biochemical identification reagent.