Magdaline Christina Rajanand, Carl Hsieh, Piyali Jana, Akshaya Panamkulangara, Sneha Jayamma Hanumanthareddy, Faseeha Akbar Shaikh, Sriraksha Srinath, Vani Rajashekaraiah
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引用次数: 0
Abstract
Background: Oxidative stress (OS) causes deleterious changes in erythrocytes leading to hemolysis and macrophage-mediated removal from the circulation. Antioxidants have proven to protect the erythrocytes from induced OS in vitro. Hydroxybenzoic acid (HBA), a phenolic compound, exhibits free radical scavenging properties. Therefore, this study was conducted to evaluate the role of HBA on erythrocytes subjected to OS. Materials and Methods: Erythrocytes isolated from the blood collected from male Wistar rats were grouped into controls and experimental groups—samples with 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), HBA10 (10 mM HBA), HBA30 (30 mM HBA), and samples with combination of AAPH and HBA. The markers of OS and antioxidant status were determined. Statistical analyses were performed using GraphPad Prism 8 Software. Results: Hemoglobin decreased in HBA and AAPH + HBA groups against their respective controls. Conjugate dienes increased in HBA10 against control. Thiobarbituric acid reactive substances (TBARSs) increased in AAPH + HBA10 against AAPH. Superoxide dismutase activity elevated in the AAPH + HBA10 group compared with AAPH. Catalase levels increased in HBA10 against control. Conclusion: p-Hydroxybenzoic acid has proved to be beneficial in scavenging the free radicals generated by AAPH as reflected in the results of hemolysis, sulfhydryls, and TBARS and antioxidant enzymes.
期刊介绍：
Applied In Vitro Toxicology is a peer-reviewed journal providing the latest research on the application of alternative in vitro testing methods for predicting adverse effects in the pharmaceutical, chemical, and personal care industries. This Journal aims to address important issues facing the various chemical industries, including regulatory requirements; the reduction, refinement, and replacement of animal testing; new screening methods; evaluation of new cell and tissue models; and the most appropriate methods for assessing safety and satisfying regulatory demands. The Journal also delivers the latest views and opinions of developers of new models, end users of the models, academic laboratories that are inventing new tools, and regulatory agencies in the United States, Europe, Latin America, Australia and Asia. Applied In Vitro Toxicology is the journal that scientists involved with hazard identification and risk assessment will read to understand how new and existing in vitro methods are applied, and the questions for which these models provide answers. Applied In Vitro Toxicology coverage includes: -Applied in vitro toxicology industry standards -New technologies developed for applied in vitro toxicology -Data acquisition, cleaning, distribution, and best practices -Data protection, privacy, and policy -Business interests from research to product -The changing role of in vitro toxicology -Visualization and design principles of applied in vitro toxicology infrastructures -Physical interfaces and robotics -Opportunities around applied in vitro toxicology
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