Esma Anissa Trad Khodja, Abd El Hamid Khabtane, Rabah Arhab, Mohamed Sabri Bensaad, Hakima Beldi, Chawki Bensouici, Ashjan A. Shami, Hamsa Jameel Banjer, Rokayya Sami, Mahmoud Helal
{"title":"Phytochemical Study, Biotechnological Applications and Experimental Evaluation of the Pharmacological Quality of <i>Tamarix Africana</i> Stems Fractions","authors":"Esma Anissa Trad Khodja, Abd El Hamid Khabtane, Rabah Arhab, Mohamed Sabri Bensaad, Hakima Beldi, Chawki Bensouici, Ashjan A. Shami, Hamsa Jameel Banjer, Rokayya Sami, Mahmoud Helal","doi":"10.1166/jbmb.2023.2309","DOIUrl":null,"url":null,"abstract":"Since antiquity, phytomedicine played a crucial role and many comprehensive texts documenting the medicinal impact of plants were discovered during many civilization eras, suggesting folk medicine is the foundation of modern medicine. This work look into the phytochemical composition, as well as the pharmacological properties, of Tamarix Africana stems organic fractions. The phytochemical study was carried out using phytochemical screening method while phenolic, flavonoids, condensed and hydrolysable tannins contents by spectrophotometric approach. The antioxidant assay was measured using five approaches. Anti-diabetic capacity was investigated with two assays. Furthermore, the cytotoxicity of the extracts was tested against Artemia Salina nauplii and the photoprotective test was assessed using sun protection factor measurement (SPF). Data revealed that both extracts contained phenolic substances, the EA fraction had the highest proportion of phenolics, flavonoids, condensed and hydrolysable tannins (825.47±2.34 μ g GAE/mg), (238.4±2.11 μ g QE/mg), (8.44±1.05 μ g CE/mg), (508.23±3.65 μ g TAE/mg) respectively. The antioxidant, antidiabetic, and cytotoxicity of these extracts evolved in dose-dependent manner. EA activity was higher than MeOH extract in antioxidant tests. A moderate antidiabetic effect was expressed by EA extract, while this effect was higher for MeOH extract. A significant cytotoxic and photoprotective effects was observed for both MeOH and EA extracts when compared to the corresponding standards. This study suggests that Tamarix Africana stems has certain pharmacological effects, especially to cure disorders linked to oxidative stress, glycemia perturbation, dermatopathology and some cancers.","PeriodicalId":15157,"journal":{"name":"Journal of Biobased Materials and Bioenergy","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biobased Materials and Bioenergy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1166/jbmb.2023.2309","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Since antiquity, phytomedicine played a crucial role and many comprehensive texts documenting the medicinal impact of plants were discovered during many civilization eras, suggesting folk medicine is the foundation of modern medicine. This work look into the phytochemical composition, as well as the pharmacological properties, of Tamarix Africana stems organic fractions. The phytochemical study was carried out using phytochemical screening method while phenolic, flavonoids, condensed and hydrolysable tannins contents by spectrophotometric approach. The antioxidant assay was measured using five approaches. Anti-diabetic capacity was investigated with two assays. Furthermore, the cytotoxicity of the extracts was tested against Artemia Salina nauplii and the photoprotective test was assessed using sun protection factor measurement (SPF). Data revealed that both extracts contained phenolic substances, the EA fraction had the highest proportion of phenolics, flavonoids, condensed and hydrolysable tannins (825.47±2.34 μ g GAE/mg), (238.4±2.11 μ g QE/mg), (8.44±1.05 μ g CE/mg), (508.23±3.65 μ g TAE/mg) respectively. The antioxidant, antidiabetic, and cytotoxicity of these extracts evolved in dose-dependent manner. EA activity was higher than MeOH extract in antioxidant tests. A moderate antidiabetic effect was expressed by EA extract, while this effect was higher for MeOH extract. A significant cytotoxic and photoprotective effects was observed for both MeOH and EA extracts when compared to the corresponding standards. This study suggests that Tamarix Africana stems has certain pharmacological effects, especially to cure disorders linked to oxidative stress, glycemia perturbation, dermatopathology and some cancers.
自古以来,植物医学就起着至关重要的作用,在许多文明时期,人们发现了许多记录植物医学影响的综合文献,这表明民间医学是现代医学的基础。这项工作着眼于植物化学成分,以及药理学性质,柽柳非洲茎有机组分。采用植物化学筛选法进行植物化学研究,采用分光光度法测定酚类、黄酮类、缩合单宁和水解单宁含量。抗氧化测定采用五种方法。用两种方法研究抗糖尿病能力。并对其进行细胞毒性试验,并采用防晒系数(SPF)评价其光防护作用。结果表明,两种提取物均含有酚类物质,其中EA部位酚类物质、黄酮类物质、缩合单宁和水解单宁含量最高,分别为(825.47±2.34 μ g GAE/mg)、(238.4±2.11 μ g QE/mg)、(8.44±1.05 μ g CE/mg)、(508.23±3.65 μ g TAE/mg)。这些提取物的抗氧化、抗糖尿病和细胞毒性呈剂量依赖性。在抗氧化试验中,EA活性高于MeOH提取物。EA提取物具有中等的降糖作用,而MeOH提取物的降糖作用更高。与相应标准相比,MeOH和EA提取物均具有显著的细胞毒和光保护作用。该研究表明,柽柳茎具有一定的药理作用,特别是对氧化应激、血糖紊乱、皮肤病理和某些癌症相关疾病的治疗。