Optimization for Extraction Proteins from Pulp of Watermelon Seed

Mağallaẗ Tikrīt li-l-ʻulūm al-zirāʻaẗ̈ Pub Date : 2023-01-18 DOI:10.25130/tjas.19.2.9

Sara Faiad, Ethar Naji, Jadooa Hajeej

引用次数: 3

Abstract

The study was carried out in the laboratories of the Faculty of Agriculture / University of Tikrit to the period 2017/11/7 - 1/5/2018. The study of watermelon seed pulp the local variety, to obtain protein isolates of the graft after removal of fat. The results showed chemical composition of the imported and local variety the percentage of moisture was 2.9%, 2.5%, ash 4.2%, 3.8%, fat 41%, 40.2%, carbohydrates 19.4%, 18.5% respectively. The result show that the percentage at important and locally were 32.5% and 35%, respectively. The watermelon seeds (imported and local) showed that the moisture 4.2% and 4%, ash 1.9% and 2.5%, fat 23.5%, 21.8%, carbohydrates 49.2%, 48.4%, protein 21.2%, 23.3% respectively. The results of this study showed that the best conditions in the preparation of protein extract from defatted pulp of watermelon seed, were by extraction with distilled water after equalized pH to 12 with ratio bran: water of 1:10 (w:v) for 60 min ,at agitation speed of 500 rpm/min, followed by sedimentation at the isoelectric point .

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西瓜籽果肉蛋白提取工艺的优化

该研究于2017年11月7日至2018年5月1日在提克里特大学农业学院的实验室进行。本研究以当地西瓜籽浆品种为研究对象，获得移植物去脂后的蛋白分离物。结果表明:进口品种和本地品种的化学成分水分含量分别为2.9%、2.5%、灰分4.2%、3.8%、脂肪41%、40.2%、碳水化合物19.4%、18.5%;结果表明，重要和局部的比例分别为32.5%和35%。进口和国产西瓜种子水分含量分别为4.2%和4%，灰分分别为1.9%和2.5%，脂肪含量分别为23.5%、21.8%，碳水化合物含量分别为49.2%、48.4%，蛋白质含量分别为21.2%、23.3%。结果表明，从西瓜籽脱脂浆中提取蛋白质的最佳工艺条件为:pH = 12，糠水比1:10 (w:v)，蒸馏水萃取60 min，搅拌速度500 rpm/min，等电点沉淀;& # x0D;

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Mağallaẗ Tikrīt li-l-ʻulūm al-zirāʻaẗ̈

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