Pathotyping and Antibiotic Resistance Characteristics of Escherichia coli Isolates from Calf Diarrhea

Abstract

Calf diarrhea is an important disease that causes economic losses and E. coli is the primary pathogen. In this study, stool samples were collected from a total of 100 calves with diarrhea. As a result of 16S rRNA PCR analysis, it was confirmed that 50 (96.15%) of 52 E. coli isolates were molecularly E. coli. As a result of molecular pathotyping studies of 50 E. coli isolates, the pathotypes of 21 (42.0%) E. coli strains were identified. E. coli (n = 50) isolates were found to be resistant to amoxicillin-clavulanate by 34%, to ciprofloxacin by 38%, and to trimethoprim-sulfamethoxazole by 60%. Different degrees of antimicrobial resistance genes were detected in 26 (52%) E. coli isolates. In conclusion, a data source was provided for the antimicrobial treatment procedure in calf diarrhea caused by E. coli in our region, and the results that could guide future studies were also brought to the literature.