Estimation of Secondary Metabolites in Gliricidia Sepium from Primary Compounds and Regrowth Age

Tatiana Gavilánez-Buñay, Danis Verdecia-Acosta, Luis Hernández-Montiel, Edilberto Chacón-Marcheco, Jorge Ramírez-de la Ribera
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Abstract

The objective is to estimate the content of secondary metabolites in Gliricidia Sepium from the age and content of primary compounds (nitrogen, fructose, glucose, and sucrose). The present study was developed following a randomized block design, with three treatments (60, 120 and 180 days) and n repetitions. Nitrogen (N), glucose (Glu), fructose (Frut) and sucrose (Suc) content were evaluated for validation. In addition, mathematical models were validated for the estimation of secondary metabolites from the primary ones and regrowth age. To establish the functional relationship between age and primary metabolites, regression equations were analyzed. Reporting a decrease for N, Glu, Frut and Suc with the highest values at 60 days, and quadratic equations with R2 greater than 0.91 were adjusted. The regrowth age had a marked effect on the contents of primary metabolites (N, Glu, Frut and Suc), which explains the close relationship through the established regression equations. As well as it was evidenced that the validation of the models for the prediction of secondary metabolites in both periods of the year, can be applied due to the dependence of these compounds on their precursors (nitrogen and sugars).
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从初代化合物和再生年龄估算黄菖蒲次生代谢物
目的是从年龄和主要化合物(氮、果糖、葡萄糖和蔗糖)的含量来估计黄菖蒲次生代谢物的含量。本研究采用随机区组设计,分为三个处理(60、120和180天)和n次重复。对氮(N)、葡萄糖(Glu)、果糖(Frut)和蔗糖(Suc)含量进行了评估。此外,通过数学模型验证了从初级代谢物和再生年龄估算次生代谢物的有效性。为了建立年龄与初级代谢物之间的函数关系,分析了回归方程。N、Glu、Frut和Suc均呈下降趋势,60 d时达到最高值,并对R2大于0.91的二次方程进行校正。再生年龄对主要代谢物(N、Glu、Frut和Suc)含量有显著影响,通过建立的回归方程可以解释二者之间的密切关系。此外,由于这些化合物依赖于它们的前体(氮和糖),因此可以在一年中的两个时期对次级代谢物的预测模型进行验证。
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