Role of sulfide anion in the development of chronic alcoholic hepatitis under the conditions of modulation of adenosine monophosphate kinase – a correlational study

Andrii Mykytenko, Oleh Akimov, Oleksandr Shevchenko, Karine Neporada
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引用次数: 1

Abstract

Introduction and aim. Hydrogen sulfide (H 2 S) has attracted the attention of researchers as a novel signaling molecule that affects vascular metabolism, immune function, stress and inflammation. It plays an important role in pathophysiological disorders under the conditions of the development of obesity, diabetes, non-alcoholic fatty liver disease and cardiovascular diseases. The purpose of this work is to establish correlation ratios of H 2 S concentration with markers of oxidative-nitrosative stress and extracellular matrix metabolism of the liver during chronic alcoholic hepatitis modeling and AMPK modulation by phenformin and doxorubicin. Material and methods. The experiments were performed on 36 white, sexually mature male Wistar rats, weighing 180-220 g. Alcoholic hepatitis was modelled by alcohol administration, on the background of alcoholic hepatitis animals received phenformin orally at a dose of 10 mg/kg or doxorubicin at a dose of 1.25 mg/kg intraperitoneally. Statistical processing of the results of biochemical studies was carried out using the non-parametric method of Spearman to determine correlations. Results. H 2 S during alcoholic hepatitis inversely proportionally strongly correlates with the concentration of nitrites, oxyproline and arginase activity. Phenformin administration during alcoholic hepatitis leads to formation of inversely proportionally strongly correlation of H 2 S with the production of superoxide anion radical, the concentration of malondialdehyde, activities of constitutive NO-synthases, nitrite reductases, nitrate reductases, and arginase. Doxorubicin administration during alcoholic hepatitis leads to formation of directly proportional strongly correlation of H 2 S with the activity of constitutive NO-synthases, nitrite reductases, nitrate reductases. Conclusion. Administration of phenformin or doxorubicin expands correlations between H 2 S and indicators of oxidative-nitrosative stress.
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腺苷单磷酸激酶调节条件下硫化物阴离子在慢性酒精性肝炎发展中的作用——一项相关研究
介绍和目的。硫化氢(h2s)作为一种影响血管代谢、免疫功能、应激和炎症的新型信号分子受到了研究人员的关注。它在肥胖、糖尿病、非酒精性脂肪肝和心血管疾病发展的病理生理障碍中起着重要作用。这项工作的目的是建立h2s浓度与慢性酒精性肝炎模型和苯双胍和阿霉素调节AMPK过程中肝脏氧化亚氧化应激和细胞外基质代谢标志物的相关比率。材料和方法。实验对象为36只性成熟雄性Wistar大鼠,体重180-220 g。在酒精性肝炎的背景下,通过酒精给药来模拟酒精性肝炎,动物口服10 mg/kg剂量的苯双胍或腹腔注射1.25 mg/kg剂量的阿霉素。采用Spearman非参数方法对生化研究结果进行统计处理,以确定相关性。结果。酒精性肝炎期间的h2s与亚硝酸盐浓度、羟脯氨酸和精氨酸酶活性成反比。酒精性肝炎期间给予苯双胍导致h2s与超氧阴离子自由基的产生、丙二醛浓度、组成型no合成酶、亚硝酸盐还原酶、硝酸盐还原酶和精氨酸酶的活性呈反比关系。酒精性肝炎患者给予阿霉素可导致h2s与组成型no合成酶、亚硝酸盐还原酶、硝酸盐还原酶活性成正比的强相关关系。结论。苯双胍或阿霉素的管理扩大了h2s和氧化-亚硝化应激指标之间的相关性。
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