Effects of Up-Regulation of Sirtuin 6 Expression on the Biological Characteristics of Non-Small Cell Lung Cancer A549 cells

Abstract

The purpose of this research was to examine the effects of sirtuin 6 overexpression on the biological characteristics of A549 cells, which are a form of non-small cell lung cancer. In the laboratory setting, the upregulation of sirtuin 6 expression in A549 cells was accomplished by introducing an overexpression plasmid (plasmid cloning deoxyribonucleic acid 3.1-sirtuin 6). Subsequently, the expression of sirtuin 6 in the transfected A549 cells was assessed through both reverse transcription-polymerase chain reaction and Western blot analysis. Proliferation was evaluated using flow cytometry, while metastatic and invasive potential were examined using the Transwell cultivation system. Additionally, apoptosis was measured utilizing the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The results revealed a significant increase (p<0.05) in sirtuin 6 expression in the plasmid cloning deoxyribonucleic acid 3.1-sirtuin 6 group compared to the control and plasmid cloning deoxyribonucleic acid 3.1 groups. Moreover, the transfection of A549 cells resulted in suppressed proliferation and invasive ability, without any noticeable impact on cell apoptosis. In conclusion, these findings demonstrate that excessive sirtuin 6 expression impedes the growth and invasive capacity of A549 cells. Targeting sirtuin 6 may represent a promising therapeutic strategy for non-small cell lung cancer treatment.