"New Perspectives on Technical Aspects of Micromanipulation (ICSI) in Clinical Assisted Reproduction"

Mohammad Ali Khalili
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Abstract

Intracytoplasmic Sperm Injection (ICSI) is presently performed for 70-80% of the ART (assisted reproductive technology) cycles [1]. This substantial use is partly due to the excessive stage of standardization through the procedure. The aim of this review was to consider the available publications dealing with the technical aspect of ICSI in assisted reproduction. The literature search was conducted employing PubMed and Google scholar. Some of the key words used: ICSI injection, ICSI outcomes, sperm immobilization, micro tools, commercial micropipette, home-made micropipette, injection speed, injection force, aspiration of cytoplasm, site of sperm deposition and laboratory set-up. The search strategy was limited to articles published in English involving human subjects, with the last search performed on June 2023.This overview provides modern-day knowledge on some technical factors to improve the ICSI efficacy in ART contexts.
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显微操作(ICSI)技术在临床辅助生殖中的新视角
目前70-80%的辅助生殖技术周期采用卵胞浆内单精子注射(ICSI)[1]。这种大量使用的部分原因是由于该程序的标准化阶段过长。本次审查的目的是考虑现有的关于辅助生殖中ICSI技术方面的出版物。使用PubMed和Google scholar进行文献检索。使用的一些关键词:ICSI注射,ICSI结果,精子固定,微工具,商用微移液器,自制微移液器,注射速度,注射力,细胞质抽吸,精子沉积位置和实验室设置。搜索策略仅限于涉及人类受试者的英文文章,最后一次搜索是在2023年6月进行的。本综述提供了一些技术因素的现代知识,以提高在ART背景下ICSI的疗效。
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