Ei Hyun Kim, Ma-Kyung Ho, Jeong Hoon Lee, Jeong-Jin Tae, Han-Jong Won, Jong Wook Chung
{"title":"Development of Genomic Simple Sequence Repeat Markers and Variety Discrimination in Salvia miltiorrhiza Bunge","authors":"Ei Hyun Kim, Ma-Kyung Ho, Jeong Hoon Lee, Jeong-Jin Tae, Han-Jong Won, Jong Wook Chung","doi":"10.7783/kjmcs.2023.31.5.304","DOIUrl":null,"url":null,"abstract":"Background: Salvia miltiorrhiza Bunge, of the Lamiaceae family, is a medicinal plant. This study aimed to develop polymorphic simple sequence repeat (SSR) markers for analyzing the genetic diversity and distinguishing between varieties of S. miltiorrhiza.BRMethods and Results: Using whole genome resequencing data, 50 SSR markers were designed for S. miltiorrhiza. Of these, 41 polymorphic markers were selected and utilized to assess 44 S. miltiorrhiza accessions. A total of 346 alleles were detected (2 to 17 per locus, averaging 8.4). Major allele frequency ranged from 0.19 to 0.83 (average 0.47), observed heterozygosity ranged from 0 to 0.60 (average 0.22), and polymorphic information content ranged from 0.29 to 0.87 (average 0.64). Among the 41 SSR markers, 18 were effective for distinguishing varieties, particularly S. miltiorrhiza ‘Dasan’, ‘Gosan’, and","PeriodicalId":22918,"journal":{"name":"The Korean Journal of Medicinal Crop Science","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Korean Journal of Medicinal Crop Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7783/kjmcs.2023.31.5.304","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Salvia miltiorrhiza Bunge, of the Lamiaceae family, is a medicinal plant. This study aimed to develop polymorphic simple sequence repeat (SSR) markers for analyzing the genetic diversity and distinguishing between varieties of S. miltiorrhiza.BRMethods and Results: Using whole genome resequencing data, 50 SSR markers were designed for S. miltiorrhiza. Of these, 41 polymorphic markers were selected and utilized to assess 44 S. miltiorrhiza accessions. A total of 346 alleles were detected (2 to 17 per locus, averaging 8.4). Major allele frequency ranged from 0.19 to 0.83 (average 0.47), observed heterozygosity ranged from 0 to 0.60 (average 0.22), and polymorphic information content ranged from 0.29 to 0.87 (average 0.64). Among the 41 SSR markers, 18 were effective for distinguishing varieties, particularly S. miltiorrhiza ‘Dasan’, ‘Gosan’, and
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