Study of the activity of antioxidant enzymes in somaclonal potato variants for use in selection for resistance to the PVS virus

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY Izvestiya Vuzov-Prikladnaya Khimiya i Biotekhnologiya Pub Date : 2023-09-30 DOI:10.21285/2227-2925-2023-13-3-442-453
I. V. Kirgizova, E. A. Kalashnikova, A. M. Gadzhimyradova, D. V. Silaev, R. M. Turpanova, S. B. Zhangazin
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Abstract

The article presents the results of a study into the activity of the antioxidant system of potatoes in response to infection with the PVS virus. The PVS virus is widespread in the Omsk region. The work set out to study the activity of antioxidant enzymes in somaclonal potato variants when infected with PVS virus to obtain valuable varietal characteristics for use in breeding selection. Potato varieties Yermak, Alena and the Khozyayushka were selected from the Omsk Agrarian Scientific Centre breeding lines as objects of research. Differences in resistance to viral infection in varieties of different susceptibility and obtained somaclones were studied using the example of changes in the activity of the isoenzyme composition of antioxidant enzymes. The results showed that somaclone EC1, which was derived from the susceptible Yermak variety, had two additional isoenzymes upon infection and an increase in isoperoxidase activity compared to the control. Although the number of isoperoxidases did not increase in the samples obtained from the stable HC94 and the moderately susceptible AC91 variety, an increase in their activity was noted. Activation occurs in infected plants of the additional 3rd isoforms of catalase compared to the control – 1 isoform, while the 4th isoform of catalase was detected in the EC1 somaclonal sample. When studying the superoxide dismutase spectra, the control group showed the activity of Mp-SOD and Fe-SOD, while two forms of the enzyme were identified in the HC94, AC91 and EC1 somaclonal samples: Feand Cu/Zn-SOD. These forms play a significant role in the neutralisation of the superoxide radical.
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马铃薯体细胞无性系变异体抗氧化酶活性的研究,用于抗PVS病毒的选择
本文介绍了马铃薯抗氧化系统对PVS病毒感染反应活性的研究结果。PVS病毒在鄂木斯克地区广泛传播。本研究旨在研究马铃薯体细胞无性系变异体感染PVS病毒后抗氧化酶的活性,以获得有价值的品种特征,为育种选择提供依据。马铃薯品种Yermak, Alena和Khozyayushka从鄂木斯克农业科学中心选育品系中选择作为研究对象。以抗氧化酶同工酶组成的活性变化为例,研究了不同易感性品种和获得的体细胞无性系对病毒感染的抗性差异。结果表明,来自Yermak易感品种的体细胞无性系EC1在感染后增加了2个同工酶,且异过氧化物酶活性较对照增加。虽然从稳定的HC94和中等敏感的AC91品种中获得的样品中异过氧化物酶的数量没有增加,但它们的活性有所增加。与对照- 1异构体相比,感染植株中出现了额外的第3种过氧化氢酶异构体的激活,而在EC1体细胞无性系样品中检测到第4种过氧化氢酶异构体。在研究超氧化物歧化酶谱时,对照组显示Mp-SOD和Fe-SOD活性,而在HC94、AC91和EC1体细胞无性系样品中鉴定出两种形式的酶:fe和Cu/Zn-SOD。这些形式在中和超氧化物自由基中起重要作用。
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来源期刊
自引率
50.00%
发文量
63
审稿时长
12 weeks
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